Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress

Overview

Journal Dose Response

Publisher Sage Publications

Date 2022 Jun 1

PMID 35645655

Authors

Fatima Mukhtar

Kashif Jilani

Ismat Bibi

Zahid Mushataq

Maham Abdul Bari Khan

Maria Fatima

Affiliations

Soon will be listed here.

Abstract

Background: Bifenthrin is an insecticide and anti-estrogenic compound primarily used to control residential pests by depolarizing sodium gated voltage channels in the nervous system. Eryptosis, the suicidal death of erythrocytes, featured by PS exposure, membrane blebbing and cell shrinkage. Anemia is an outcome of uncontrolled eryptosis.

Research Design: In this study, erythrocytes were treated with different concentrations (.5-1-1.5 μM) of bifenthrin over a period of 48 hours. In order to investigate the oxidative stress induced by bifenthrin, catalase, superoxide dismutase, and glutathione peroxidase activities were investigated.

Results: Obtained data indicated the decrease in the enzymes (superoxide dismutase, glutathione peroxidase, and catalase) activities in bifenthrin treated cells at 1 μM concentration. In addition, measurement of cell size and confirmation of the role of calcium in the stimulation of the eryptotic activity of bifenthrin were performed. A significant increase in mean cell volume was found in the presence of bifenthrin and a decrease in mean cell volume in the presence of calcium channel blocker was observed. Similarly, there was also a significant increase in the percentage of hemolysis indicating the necrotic activity of bifenthrin.

Conclusions: It is concluded that the indicated doses of bifenthrin triggered oxidative stress which may lead to early cell death by eryptosis and hemolysis.

Citing Articles

Levofloxacin induces erythrocyte contraction leading to red cell death.

Aslam H, Sohail A, Shahid A, Khan M, Sharif M, Kausar R Drug Target Insights. 2024; 18:78-83.

PMID: 39386351 PMC: 11462248. DOI: 10.33393/dti.2024.3060.

Apoptosis and eryptosis: similarities and differences.

Tkachenko A Apoptosis. 2023; 29(3-4):482-502.

PMID: 38036865 DOI: 10.1007/s10495-023-01915-4.

References

DOnofrio G, Chirillo R, Zini G, Caenaro G, Tommasi M, Micciulli G . Simultaneous measurement of reticulocyte and red blood cell indices in healthy subjects and patients with microcytic and macrocytic anemia. Blood. 1995; 85(3):818-23. View

Lang E, Jilani K, Bissinger R, Rexhepaj R, Zelenak C, Lupescu A . Vitamin D-Rich Diet in Mice Modulates Erythrocyte Survival. Kidney Blood Press Res. 2015; 40(4):403-12. DOI: 10.1159/000368517. View

Lupescu A, Bissinger R, Jilani K, Lang F . Triggering of suicidal erythrocyte death by celecoxib. Toxins (Basel). 2013; 5(9):1543-54. PMC: 3798872. DOI: 10.3390/toxins5091543. View

Zbidah M, Lupescu A, Yang W, Bosc A, Jilani K, Shaik N . Sulindac sulfide--induced stimulation of eryptosis. Cell Physiol Biochem. 2012; 30(4):1072-82. DOI: 10.1159/000341483. View

Yousef M, Awad T, Mohamed E . Deltamethrin-induced oxidative damage and biochemical alterations in rat and its attenuation by Vitamin E. Toxicology. 2006; 227(3):240-7. DOI: 10.1016/j.tox.2006.08.008. View

Lupescu A, Bissinger R, Warsi J, Jilani K, Lang F . Stimulation of erythrocyte cell membrane scrambling by gedunin. Cell Physiol Biochem. 2014; 33(6):1838-48. DOI: 10.1159/000362962. View

Ilyas S, Jilani K, Sikandar M, Siddiq S, Riaz M, Naveed A . Stimulation of Erythrocyte Membrane Blebbing by Naproxen Sodium. Dose Response. 2020; 18(1):1559325819899259. PMC: 6961146. DOI: 10.1177/1559325819899259. View

Rapido F . The potential adverse effects of haemolysis. Blood Transfus. 2017; 15(3):218-221. PMC: 5448827. DOI: 10.2450/2017.0311-16. View

Birben E, Sahiner U, Sackesen C, Erzurum S, Kalayci O . Oxidative stress and antioxidant defense. World Allergy Organ J. 2012; 5(1):9-19. PMC: 3488923. DOI: 10.1097/WOX.0b013e3182439613. View

10.

Naveed A, Jilani K, Siddique A, Akbar M, Riaz M, Mushtaq Z . Induction of Erythrocyte Shrinkage by Omeprazole. Dose Response. 2020; 18(3):1559325820946941. PMC: 7432979. DOI: 10.1177/1559325820946941. View

11.

El-Demerdash F . Oxidative stress and hepatotoxicity induced by synthetic pyrethroids-organophosphate insecticides mixture in rat. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2011; 29(2):145-58. DOI: 10.1080/10590501.2011.577679. View

12.

Gammon D, Liu Z, Chandrasekaran A, ElNaggar S . The pharmacokinetic properties of bifenthrin in the rat following multiple routes of exposure. Pest Manag Sci. 2014; 71(6):835-41. DOI: 10.1002/ps.3883. View

13.

Bissinger R, Mamun Bhuyan A, Qadri S, Lang F . Oxidative stress, eryptosis and anemia: a pivotal mechanistic nexus in systemic diseases. FEBS J. 2018; 286(5):826-854. DOI: 10.1111/febs.14606. View

14.

Hassan M, Hadi R, Al-Rawi Z, Padron V, Stohs S . The glutathione defense system in the pathogenesis of rheumatoid arthritis. J Appl Toxicol. 2001; 21(1):69-73. DOI: 10.1002/jat.736. View

15.

Kerr J . History of the events leading to the formulation of the apoptosis concept. Toxicology. 2002; 181-182:471-4. DOI: 10.1016/s0300-483x(02)00457-2. View

16.

Abraham S, Soundararajan C, Vivekanandhan S, Behari M . Erythrocyte antioxidant enzymes in Parkinson's disease. Indian J Med Res. 2005; 121(2):111-5. View

17.

Rana R, Jilani K, Shahid M, Riaz M, Ranjha M, Bibi I . Atorvastatin Induced Erythrocytes Membrane Blebbing. Dose Response. 2019; 17(3):1559325819869076. PMC: 6691667. DOI: 10.1177/1559325819869076. View

18.

Dar M, Khan A, Raina R, Verma P, Wani N . Effect of bifenthrin on oxidative stress parameters in the liver, kidneys, and lungs of rats. Environ Sci Pollut Res Int. 2019; 26(9):9365-9370. DOI: 10.1007/s11356-019-04362-4. View

19.

Soderlund D . Molecular mechanisms of pyrethroid insecticide neurotoxicity: recent advances. Arch Toxicol. 2011; 86(2):165-81. PMC: 3218237. DOI: 10.1007/s00204-011-0726-x. View

20.

Gargouri B, Yousif N, Bouchard M, Fetoui H, Fiebich B . Inflammatory and cytotoxic effects of bifenthrin in primary microglia and organotypic hippocampal slice cultures. J Neuroinflammation. 2018; 15(1):159. PMC: 5968622. DOI: 10.1186/s12974-018-1198-1. View