Heat Stress Response in Chinese Cabbage ( L.) Revealed by Transcriptome and Physiological Analysis

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2022 May 31

PMID 35637719

Authors

Lei Zhang

Yun Dai

Lixin Yue

Guohu Chen

Lingyun Yuan

Shifan Zhang

Fei Li

Hui Zhang

Guoliang Li

Shidong Zhu

Jinfeng Hou

Xiaoyan Tang

Shujiang Zhang

Chenggang Wang

Affiliations

Soon will be listed here.

Abstract

High temperatures have a serious impact on the quality and yield of cold-loving Chinese cabbage, which has evolved to have a unique set of stress mechanisms. To explore the relationship between these mechanisms and the heat-tolerance of Chinese cabbage, the physiological indicators of the heat-tolerant '268' line and heat-sensitive '334' line were measured. Under heat stress, the proline (Pro), soluble sugar (SS), and superoxide dismutase (SOD) indexes of the '268' line increased significantly. When additionally using transcriptome analysis, we found that the identified 3,360 DEGs were abundantly enriched in many metabolic pathways including 'plant hormone signal transduction', 'carbon metabolism', and 'glycolysis/gluconeogenesis'. Dynamic gene expression patterns showed that in Cluster 15 may be a key factor in the regulation of sugar homeostasis. The interaction network screened four ABA-related genes in Cluster 15, suggesting that high temperatures lead to changes in hormonal signaling, especially an increase in ABA signaling. Compared with the '334' line, the expressions of , , , , and in the '268' line were significantly upregulated, and these genes were actively involved in the reactive oxygen species (ROS) scavenging process. In summary, our results revealed the relationship between plant heat tolerance, physiology, and biochemistry and may also provide ideas for the future development of high-quality and heat-tolerant Chinese cabbage germplasm resources.

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