Genetic Polymorphisms of in HIV-Positive and HIV-Negative Patients in Northern China

Overview

Journal Pol J Microbiol

Specialty Microbiology

Date 2022 May 31

PMID 35635165

Authors

Ting Xue

Wei-Qin Du

Wen-Juan Dai

Yi-Shan Li

Shu-Feng Wang

Jun-Ling Wang

Xin-Ri Zhang

Affiliations

Soon will be listed here.

Abstract

is an opportunistic fungus that can cause severe and potentially fatal pneumonia (PCP) in immunodeficient patients. In this study, we investigated the genetic polymorphisms of at eight different loci, including six nuclear genes (ITS, 26S rRNA, , , and β-Tub) and two mitochondrial genes (mtLSU-rRNA and ) in three PCP cases, including two patients with HIV infection and one without HIV infection in Shanxi Province, P.R. China. The gene targets were amplified by PCR followed by sequencing of plasmid clones. The HIV-negative patient showed a coinfection with two genotypes of at six of the eight loci sequenced. Of the two HIV-positive patients, one showed a coinfection with two genotypes of at the same two of the six loci as in the HIV-negative patient, while the other showed a single infection at all eight loci sequenced. None of the three drug target genes ( and ) showed mutations known to be potentially associated with drug resistance. This is the first report of genetic polymorphisms of in PCP patients in Shanxi Province, China. Our findings expand our understanding of the genetic diversity of in China.

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