De Vriese Ameliorates LPS-Induced Acute Lung Injury Via Src Inhibition

Overview

Journal Plants (Basel)

Date 2022 May 28

PMID 35631731

Authors

Won Young Jang

Hwa Pyoung Lee

Seung A Kim

Lei Huang

Ji Hye Yoon

Chae Yun Shin

Ankita Mitra

Han Gyung Kim

Jae Youl Cho

Affiliations

Soon will be listed here.

Abstract

Growing demand for treatment options against acute lung injury (ALI) emphasizes studies on plant extracts harboring anti-inflammatory effects. According to GC-MS analysis, de Vriese consists of various flavonoids with anti-inflammatory activities. Thus, in this study, the anti-inflammatory effects of an extract of de Vriese (Ac-EE) were assessed using RAW264.6 murine macrophages and a lipopolysaccharide (LPS)-induced ALI model. Ac-EE reduced the nitric oxide production in murine macrophages increased by LPS induction. Moreover, protective effects of Ac-EE on lung tissue were demonstrated by shrinkage of edema and lung injury. Reduced neutrophil infiltration and formation of hyaline membranes were also detected in lung tissues after H&E staining. Semiquantitative RT-PCR, quantitative real-time PCR, and ELISA showed that Ac-EE inhibits the production of proinflammatory mediators, including NOS and COX-2, and cytokines, such as TNF-α, IL-1β, and IL-6. An Ac-EE-mediated anti-inflammatory response was derived from inhibiting the NF-κB signaling pathway, which was evaluated by luciferase reporter assay and Western blotting analysis. A cellular thermal shift assay revealed that the prime target of Ac-EE in alleviating inflammation was Src. With its direct binding with Src, de Vriese significantly mitigates lung injury, showing possibilities of its potential as an effective botanical drug.

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