Influence of 40 Hz and 100 Hz Vibration on SH-SY5Y Cells Growth and Differentiation-A Preliminary Study

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2022 May 28

PMID 35630814

Authors

Patrycja Grosman-Dziewiszek

Benita Wiatrak

Wojciech Dziewiszek

Paulina Jawien

Remigiusz Mydlikowski

Romuald Bolejko

Marta Szandruk-Bender

Ewa Karuga-Kuzniewska

Adam Szelag

Affiliations

Soon will be listed here.

Abstract

(1) Background: A novel bioreactor platform of neuronal cell cultures using low-magnitude, low-frequency (LMLF) vibrational stimulation was designed to discover vibration influence and mimic the dynamic environment of the in vivo state. To better understand the impact of 40 Hz and 100 Hz vibration on cell differentiation, we join biotechnology and advanced medical technology to design the nano-vibration system. The influence of vibration on the development of nervous tissue on the selected cell line SH-SY5Y (experimental research model in Alzheimer's and Parkinson's) was investigated. (2) Methods: The vibration stimulation of cell differentiation and elongation of their neuritis were monitored. We measured how vibrations affect the morphology and differentiation of nerve cells in vitro. (3) Results: The highest average length of neurites was observed in response to the 40 Hz vibration on the collagen surface in the differentiating medium, but cells response did not increase with vibration frequency. Also, vibrations at a frequency of 40 Hz or 100 Hz did not affect the average density of neurites. 100 Hz vibration increased the neurites density significantly with time for cultures on collagen and non-collagen surfaces. The exposure of neuronal cells to 40 Hz and 100 Hz vibration enhanced cell differentiation. The 40 Hz vibration has the best impact on neuronal-like cell growth and differentiation. (4) Conclusions: The data demonstrated that exposure to neuronal cells to 40 Hz and 100 Hz vibration enhanced cell differentiation and proliferation. This positive impact of vibration can be used in tissue engineering and regenerative medicine. It is planned to optimize the processes and study its molecular mechanisms concerning carrying out the research.

Citing Articles

Brain Disease-Modifying Effects of Radiofrequency as a Non-Contact Neuronal Stimulation Technology.

Sun S, Bok J, Jang Y, Seo H Int J Mol Sci. 2025; 26(5).

PMID: 40076887 PMC: 11900402. DOI: 10.3390/ijms26052268.

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