The Rice Malectin Regulates Plant Cell Death and Disease Resistance by Participating in Glycoprotein Quality Control

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 28

PMID 35628631

Authors

Huijing Feng

Tiancheng Qiu

Changfa Yin

Xiaosheng Zhao

Guangyuan Xu

Linlu Qi

Yan Zhang

Youliang Peng

Wensheng Zhao

Affiliations

Soon will be listed here.

Abstract

In animals, malectin is well known to play an essential role in endoplasmic reticulum quality control (ERQC) by interacting with ribophorin I, one unit of the oligosaccharyltransferase (OST) complex. However, the functions of malectin in plants remain largely unknown. Here, we demonstrate the rice is an ER- and Golgi-associated malectin protein and physically interacts with rice homolog of ribophorin I (OsRpn1), and its disruption leads to spontaneous lesion mimic lesions, enhanced disease resistance, and prolonged ER stress. In addition, there are many more -glycosites and -glycoproteins identified from the mutant than wildtype. Furthermore, OsSERK1 and OsSERK2, which have more -glycosites in , were demonstrated to interact with OsMLD1. can suppress OsSERK1- or OsSERK2-induced cell death. Thus, may play a similar role to its mammalian homologs in glycoprotein quality control, thereby regulating cell death and immunity of rice, which uncovers the function of malectin in plants.

Citing Articles

Exploring the Structural Diversity and Biotechnological Potential of the Rhodophyte Phycolectome.

Rodrigues E, Verza F, Nishimura F, Beleboni R, Hermans C, Janssens K Mar Drugs. 2025; 23(1).

PMID: 39852510 PMC: 11766507. DOI: 10.3390/md23010008.

A transporter's doom or destiny: in health and disease, novel molecular targets and emerging therapeutic prospects.

Shah N, Kasture A, Fischer F, Sitte H, Hummel T, Sucic S Front Mol Neurosci. 2024; 17:1466694.

PMID: 39268250 PMC: 11390516. DOI: 10.3389/fnmol.2024.1466694.

Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall.

Oelmuller R, Tseng Y, Gandhi A Int J Mol Sci. 2023; 24(8).

PMID: 37108585 PMC: 10139151. DOI: 10.3390/ijms24087417.

Malectin Domain Protein Kinase (MDPK) Promotes Rice Resistance to Sheath Blight via IDD12, IDD13, and IDD14.

Cui Z, Xue C, Mei Q, Xuan Y Int J Mol Sci. 2022; 23(15).

PMID: 35897795 PMC: 9331740. DOI: 10.3390/ijms23158214.

References

Wan S, Jiang L . Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) in plants. Protoplasma. 2015; 253(3):753-764. DOI: 10.1007/s00709-015-0842-1. View

Yang Q, Fu M, Gao H, Yamamoto K, Hu D, Qin S . Subcellular distribution of endogenous malectin under rest and stress conditions is regulated by ribophorin I. Glycobiology. 2018; 28(6):374-381. DOI: 10.1093/glycob/cwy034. View

Liebrand T, Smit P, Abd-El-Haliem A, de Jonge R, Cordewener J, America A . Endoplasmic reticulum-quality control chaperones facilitate the biogenesis of Cf receptor-like proteins involved in pathogen resistance of tomato. Plant Physiol. 2012; 159(4):1819-33. PMC: 3425215. DOI: 10.1104/pp.112.196741. View

Fradin E, Abd-El-Haliem A, Masini L, van den Berg G, Joosten M, Thomma B . Interfamily transfer of tomato Ve1 mediates Verticillium resistance in Arabidopsis. Plant Physiol. 2011; 156(4):2255-65. PMC: 3149960. DOI: 10.1104/pp.111.180067. View

Qin P, Fan S, Deng L, Zhong G, Zhang S, Li M . LML1, Encoding a Conserved Eukaryotic Release Factor 1 Protein, Regulates Cell Death and Pathogen Resistance by Forming a Conserved Complex with SPL33 in Rice. Plant Cell Physiol. 2018; 59(5):887-902. DOI: 10.1093/pcp/pcy056. View

Galli C, Bernasconi R, Solda T, Calanca V, Molinari M . Malectin participates in a backup glycoprotein quality control pathway in the mammalian ER. PLoS One. 2011; 6(1):e16304. PMC: 3027649. DOI: 10.1371/journal.pone.0016304. View

Balint-Kurti P . The plant hypersensitive response: concepts, control and consequences. Mol Plant Pathol. 2019; 20(8):1163-1178. PMC: 6640183. DOI: 10.1111/mpp.12821. View

Apweiler R, Hermjakob H, Sharon N . On the frequency of protein glycosylation, as deduced from analysis of the SWISS-PROT database. Biochim Biophys Acta. 1999; 1473(1):4-8. DOI: 10.1016/s0304-4165(99)00165-8. View

de Oliveira M, Xu G, Li B, de Souza Vespoli L, Meng X, Chen X . Specific control of Arabidopsis BAK1/SERK4-regulated cell death by protein glycosylation. Nat Plants. 2016; 2:15218. PMC: 5572757. DOI: 10.1038/nplants.2015.218. View

10.

Zhao J, Liu P, Li C, Wang Y, Guo L, Jiang G . LMM5.1 and LMM5.4, two eukaryotic translation elongation factor 1A-like gene family members, negatively affect cell death and disease resistance in rice. J Genet Genomics. 2017; 44(2):107-118. DOI: 10.1016/j.jgg.2016.12.005. View

11.

Tan G, Gao Y, Shi M, Zhang X, He S, Chen Z . SiteFinding-PCR: a simple and efficient PCR method for chromosome walking. Nucleic Acids Res. 2005; 33(13):e122. PMC: 1182332. DOI: 10.1093/nar/gni124. View

12.

Yuchun R, Ran J, Sheng W, Xianmei W, Hanfei Y, Chenyang P . SPL36 Encodes a Receptor-like Protein Kinase that Regulates Programmed Cell Death and Defense Responses in Rice. Rice (N Y). 2021; 14(1):34. PMC: 8026784. DOI: 10.1186/s12284-021-00475-y. View

13.

Van Hautegem T, Waters A, Goodrich J, Nowack M . Only in dying, life: programmed cell death during plant development. Trends Plant Sci. 2014; 20(2):102-13. DOI: 10.1016/j.tplants.2014.10.003. View

14.

Ma J, Wang Y, Ma X, Meng L, Jing R, Wang F . Disruption of gene SPL35, encoding a novel CUE domain-containing protein, leads to cell death and enhanced disease response in rice. Plant Biotechnol J. 2019; 17(8):1679-1693. PMC: 6662554. DOI: 10.1111/pbi.13093. View

15.

Saur I, Bauer S, Lu X, Schulze-Lefert P . A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors. Plant Methods. 2019; 15:118. PMC: 6813131. DOI: 10.1186/s13007-019-0502-0. View

16.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

17.

Wang L, Pei Z, Tian Y, He C . OsLSD1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. Mol Plant Microbe Interact. 2005; 18(5):375-84. DOI: 10.1094/MPMI-18-0375. View

18.

Qiang X, Zechmann B, Reitz M, Kogel K, Schafer P . The mutualistic fungus Piriformospora indica colonizes Arabidopsis roots by inducing an endoplasmic reticulum stress-triggered caspase-dependent cell death. Plant Cell. 2012; 24(2):794-809. PMC: 3315247. DOI: 10.1105/tpc.111.093260. View

19.

Fekih R, Tamiru M, Kanzaki H, Abe A, Yoshida K, Kanzaki E . The rice (Oryza sativa L.) LESION MIMIC RESEMBLING, which encodes an AAA-type ATPase, is implicated in defense response. Mol Genet Genomics. 2014; 290(2):611-22. DOI: 10.1007/s00438-014-0944-z. View

20.

Qin C, Li Y, Gan J, Wang W, Zhang H, Liu Y . OsDGL1, a homolog of an oligosaccharyltransferase complex subunit, is involved in N-glycosylation and root development in rice. Plant Cell Physiol. 2012; 54(1):129-37. DOI: 10.1093/pcp/pcs159. View