Adverse Effects of Single Neutrophil Extracellular Trap-Derived Components on Bovine Sperm Function

Overview

Journal Animals (Basel)

Date 2022 May 28

PMID 35625154

Authors

Claudia Moya

Rodrigo Rivera-Concha

Felipe Pezo

Pamela Uribe

Mabel Schulz

Raul Sanchez

Carlos Hermosilla

Anja Taubert

Ulrich Gartner

Fabiola Zambrano

Affiliations

Soon will be listed here.

Abstract

Neutrophil extracellular traps (NETs) play a key role in fertilisation by eliminating microorganisms and entrapping spermatozoa in the female reproductive tract (FRT). The deleterious effects of NETs on spermatozoa have been previously described; however, individual exposure to NET-derived components in bull spermatozoa has not been explored. The aim of this study was to evaluate the effects of the main NET-derived proteins, histone 2A (H2A), neutrophil elastase (ELA), myeloperoxidase (MPO), pentraxin 3 (PTX), cathepsin G (Cat-G), and cathelicidin LL37 (LL-37), at concentrations of 1, 10, and 30 μg/mL, on sperm parameters. Sperm were selected and incubated with different NET-derived proteins for 4 h. Membrane and acrosome integrity, lipoperoxidation, and membrane phospholipid disorders were also evaluated. Bovine polymorphonuclear neutrophil (PMN)/sperm co-cultures were evaluated by scanning electron microscopy and immunofluorescence. All NET-derived proteins/enzymes resulted in a reduction in membrane integrity, acrosome integrity, and lipoperoxidation at a concentration of 30 μg/mL. Bovine PMN/sperm co-cultures showed marked NET formation in the second hour. In conclusion, all NET-derived proteins/enzymes exerted cytotoxic effects on bull sperm, and this effect should be considered in future investigations on the uterine microenvironment and the advancement of spermatozoa in the FRT.

Citing Articles

Cytotoxic Oxidative Stress Effects of Neutrophil Extracellular Traps' Components on Cattle Spermatozoa.

Rivera-Concha R, Leon M, Prado-Sanhueza A, Sanchez R, Taubert A, Hermosilla C Antioxidants (Basel). 2024; 13(6).

PMID: 38929172 PMC: 11200964. DOI: 10.3390/antiox13060733.

Extrusion of Neutrophil Extracellular Traps (NETs) Negatively Impacts Canine Sperm Functions: Implications in Reproductive Failure.

Leon M, Moya C, Rivera-Concha R, Pezo F, Uribe P, Schulz M Int J Mol Sci. 2024; 25(11).

PMID: 38892404 PMC: 11172674. DOI: 10.3390/ijms25116216.

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