Single Extracellular Vesicle Analysis for Early Cancer Detection

Overview

Journal Trends Mol Med

Specialties General Medicine
Molecular Biology

Date 2022 May 27

PMID 35624008

Authors

Scott Ferguson

Katherine S Yang

Ralph Weissleder

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are actively shed into the circulation from both cancer and host cells. EVs are emerging as one of the diagnostic frontrunners for early cancer detection, disease monitoring, and treatment evaluation. The advantages of EVs rely on the fact that vesicles are being shed by dividing tumor cells, with indications that human and viral oncogenes, cellular metabolic rate, and tumor characteristics such as pH and hypoxia contribute to the high shed rates in cancer. In this review, we provide an overview of EVs and the rationale for using them for early cancer detection. We examine emerging technologies for single EV analysis (sEVA) and why these technologies will be necessary for early cancer detection.

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References

Best M, Sol N, In t Veld S, Vancura A, Muller M, Niemeijer A . Swarm Intelligence-Enhanced Detection of Non-Small-Cell Lung Cancer Using Tumor-Educated Platelets. Cancer Cell. 2017; 32(2):238-252.e9. PMC: 6381325. DOI: 10.1016/j.ccell.2017.07.004. View

Stremersch S, Marro M, Pinchasik B, Baatsen P, Hendrix A, De Smedt S . Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy. Small. 2016; 12(24):3292-301. DOI: 10.1002/smll.201600393. View

Patel G, Khan M, Zubair H, Srivastava S, Khushman M, Singh S . Comparative analysis of exosome isolation methods using culture supernatant for optimum yield, purity and downstream applications. Sci Rep. 2019; 9(1):5335. PMC: 6441044. DOI: 10.1038/s41598-019-41800-2. View

Ferguson S, Weissleder R . Modeling EV Kinetics for Use in Early Cancer Detection. Adv Biosyst. 2020; 4(12):e1900305. PMC: 7658022. DOI: 10.1002/adbi.201900305. View

Guerreiro E, Vestad B, Steffensen L, Aass H, Saeed M, Ovstebo R . Efficient extracellular vesicle isolation by combining cell media modifications, ultrafiltration, and size-exclusion chromatography. PLoS One. 2018; 13(9):e0204276. PMC: 6160036. DOI: 10.1371/journal.pone.0204276. View

Han C, Kang H, Yi J, Kang M, Lee H, Kwon Y . Single-vesicle imaging and co-localization analysis for tetraspanin profiling of individual extracellular vesicles. J Extracell Vesicles. 2021; 10(3):e12047. PMC: 7797949. DOI: 10.1002/jev2.12047. View

Conde-Vancells J, Rodriguez-Suarez E, Embade N, Gil D, Matthiesen R, Valle M . Characterization and comprehensive proteome profiling of exosomes secreted by hepatocytes. J Proteome Res. 2009; 7(12):5157-66. PMC: 2696236. DOI: 10.1021/pr8004887. View

Yang Z, LaRiviere M, Ko J, Till J, Christensen T, Yee S . A Multianalyte Panel Consisting of Extracellular Vesicle miRNAs and mRNAs, cfDNA, and CA19-9 Shows Utility for Diagnosis and Staging of Pancreatic Ductal Adenocarcinoma. Clin Cancer Res. 2020; 26(13):3248-3258. PMC: 7334066. DOI: 10.1158/1078-0432.CCR-19-3313. View

Ko J, Wang Y, Carlson J, Marquard A, Gungabeesoon J, Charest A . Single Extracellular Vesicle Protein Analysis Using Immuno-Droplet Digital Polymerase Chain Reaction Amplification. Adv Biosyst. 2020; 4(12):e1900307. PMC: 8491538. DOI: 10.1002/adbi.201900307. View

10.

Fathi M, Joseph R, Adolacion J, Martinez-Paniagua M, An X, Gabrusiewicz K . Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles. Cancers (Basel). 2021; 13(17). PMC: 8430892. DOI: 10.3390/cancers13174397. View

11.

Poupardin R, Wolf M, Strunk D . Adherence to minimal experimental requirements for defining extracellular vesicles and their functions. Adv Drug Deliv Rev. 2021; 176:113872. DOI: 10.1016/j.addr.2021.113872. View

12.

Nikoloff J, Saucedo-Espinosa M, Kling A, Dittrich P . Identifying extracellular vesicle populations from single cells. Proc Natl Acad Sci U S A. 2021; 118(38). PMC: 8463870. DOI: 10.1073/pnas.2106630118. View

13.

Roweth H, Battinelli E . Lessons to learn from tumor-educated platelets. Blood. 2021; 137(23):3174-3180. PMC: 8351883. DOI: 10.1182/blood.2019003976. View

14.

Best M, Wesseling P, Wurdinger T . Tumor-Educated Platelets as a Noninvasive Biomarker Source for Cancer Detection and Progression Monitoring. Cancer Res. 2018; 78(13):3407-3412. DOI: 10.1158/0008-5472.CAN-18-0887. View

15.

Parisse P, Rago I, Ulloa Severino L, Perissinotto F, Ambrosetti E, Paoletti P . Atomic force microscopy analysis of extracellular vesicles. Eur Biophys J. 2017; 46(8):813-820. DOI: 10.1007/s00249-017-1252-4. View

16.

Wan Y, Liu B, Lei H, Zhang B, Wang Y, Huang H . Nanoscale extracellular vesicle-derived DNA is superior to circulating cell-free DNA for mutation detection in early-stage non-small-cell lung cancer. Ann Oncol. 2018; 29(12):2379-2383. PMC: 6311950. DOI: 10.1093/annonc/mdy458. View

17.

Cohen J, Li L, Wang Y, Thoburn C, Afsari B, Danilova L . Detection and localization of surgically resectable cancers with a multi-analyte blood test. Science. 2018; 359(6378):926-930. PMC: 6080308. DOI: 10.1126/science.aar3247. View

18.

Chin L, Son T, Hong J, Liu A, Skog J, Castro C . Plasmonic Sensors for Extracellular Vesicle Analysis: From Scientific Development to Translational Research. ACS Nano. 2020; 14(11):14528-14548. PMC: 8423498. DOI: 10.1021/acsnano.0c07581. View

19.

Ko J, Bhagwat N, Black T, Yee S, Na Y, Fisher S . miRNA Profiling of Magnetic Nanopore-Isolated Extracellular Vesicles for the Diagnosis of Pancreatic Cancer. Cancer Res. 2018; 78(13):3688-3697. DOI: 10.1158/0008-5472.CAN-17-3703. View

20.

Kim D, Woo H, Lee C, Min Y, Kumar S, Sunkara V . EV-Ident: Identifying Tumor-Specific Extracellular Vesicles by Size Fractionation and Single-Vesicle Analysis. Anal Chem. 2020; 92(8):6010-6018. DOI: 10.1021/acs.analchem.0c00285. View