Molecular and Functional Characterization of Peptidoglycan Recognition Proteins OfPGRP-A and OfPGRP-B in (Lepidoptera: Crambidae)

Overview

Journal Insects

Specialty Biology

Date 2022 May 27

PMID 35621753

Authors

Zengxia Wang

Wan Zhou

Baohong Huang

Mengyuan Gao

Qianqian Li

Yidong Tao

Zhenying Wang

Affiliations

Soon will be listed here.

Abstract

Peptidoglycan recognition proteins (PGRPs) are important components of insect immune systems, in which they play key roles. We cloned and sequenced two full-length PGRP, named and , from the Asian corn borer, . These two genes comprise open reading frames of 658 and 759 bp, encoding proteins of 192 and 218 amino acids, respectively. qPCR showed that and are prominently expressed in the midgut of fourth instar larvae. After inoculation with and , the expression of was significantly upregulated, whereas the expression of was enhanced after inoculation with Escherichia coli. This suggests that mainly recognizes Gram-positive bacteria and may participate in the Toll signaling pathways, while identifies Gram-negative bacteria and may participate in Imd signaling pathways. Our results provide insights into the roles of PGRPs in immune function and a foundation for using pathogens for the biological control of .

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