MatriGrid Based Biological Morphologies: Tools for 3D Cell Culturing

Overview

Journal Bioengineering (Basel)

Date 2022 May 27

PMID 35621498

Authors

Patrick Mai

Jorg Hampl

Martin Baca

Dana Brauer

Sukhdeep Singh

Frank Weise

Justyna Borowiec

Andre Schmidt

Johanna Merle Kustner

Maren Klett

Michael Gebinoga

Insa S Schroeder

Udo R Markert

Felix Glahn

Berit Schumann

Diana Eckstein

Andreas Schober

Affiliations

Soon will be listed here.

Abstract

Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrids. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account.

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