Asiatic Acid Re-sensitizes Multidrug-resistant A549/DDP Cells to Cisplatin by Down Regulating Long Non-coding RNA Metastasis Associated Lung Adenocarcinoma Transcript 1/β-catenin Signaling

Overview

Journal Bioengineered

Date 2022 May 24

PMID 35609308

Authors

Qilai Cheng

Shanshan Zhang

Bing Zhong

Zhixi Chen

Fang Peng

Affiliations

Soon will be listed here.

Abstract

Drug resistance becomes a challenge in the therapeutic management of non-small cell lung cancer (NSCLC). According to our former research, asiatic acid (AA) re-sensitized A549/DDP cells to cisplatin (DDP) through decreasing multidrug resistance protein 1 (MDR1) expression level. However, the relevant underlying mechanisms are still unclear. Long non-coding RNA (lncRNA) MALAT1 shows close association with chemo-resistance. As reported in this research, AA increased apoptosis rate, down regulated the expression of MALAT1, p300, β-catenin, and MDR1, up regulated the expression of miR-1297, and decreased β-catenin nuclear translocation in A549/DDP cells. MALAT1 knockdown expression abolished the drug resistance of A549/DDP cells and increased cell apoptosis. MALAT1 could potentially produce interactions with miR-1297, which targeted to degradation of p300. In addition, p300 overexpression effectively rescued the effects of MALAT1 knockdown expression on A549/DDP cells and activate the expression of β-catenin/MDR1 signaling, and these could be effectively blocked by AA treatment. Conclusively, AA could re-sensitize A549/DDP cells to DDP through down-regulating MALAT1/miR-1297/p300/β-catenin signaling.

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References

Liu Y, Zhuang H, Cao F, Li J, Guo Y, Zhang J . Shc3 promotes hepatocellular carcinoma stemness and drug resistance by interacting with β-catenin to inhibit its ubiquitin degradation pathway. Cell Death Dis. 2021; 12(3):278. PMC: 7961052. DOI: 10.1038/s41419-021-03560-8. View

Kypta R, Waxman J . Wnt/β-catenin signalling in prostate cancer. Nat Rev Urol. 2012; 9(8):418-28. DOI: 10.1038/nrurol.2012.116. View

Yang D, Feng W, Zhuang Y, Liu J, Feng Z, Xu T . Long non-coding RNA linc00665 inhibits CDKN1C expression by binding to EZH2 and affects cisplatin sensitivity of NSCLC cells. Mol Ther Nucleic Acids. 2021; 23:1053-1065. PMC: 7887328. DOI: 10.1016/j.omtn.2021.01.013. View

Ono M, Lai K, Wu K, Nguyen C, Lin D, Murali R . Nuclear receptor/Wnt beta-catenin interactions are regulated via differential CBP/p300 coactivator usage. PLoS One. 2018; 13(7):e0200714. PMC: 6051640. DOI: 10.1371/journal.pone.0200714. View

Gou X, Bai H, Liu L, Chen H, Shi Q, Chang L . Asiatic Acid Interferes with Invasion and Proliferation of Breast Cancer Cells by Inhibiting WAVE3 Activation through PI3K/AKT Signaling Pathway. Biomed Res Int. 2020; 2020:1874387. PMC: 7035546. DOI: 10.1155/2020/1874387. View

Garg M, Maurya N . WNT/β-catenin signaling in urothelial carcinoma of bladder. World J Nephrol. 2019; 8(5):83-94. PMC: 6794554. DOI: 10.5527/wjn.v8.i5.83. View

Wu W, Yang J, Wang Y, Wang H, Yao M, Wang L . Reversal of multidrug resistance of hepatocellular carcinoma cells by metformin through inhibiting NF-κB gene transcription. World J Hepatol. 2016; 8(23):985-93. PMC: 4990762. DOI: 10.4254/wjh.v8.i23.985. View

Islam M, Ali E, Uddin S, Khan I, Shill M, de Castro E Sousa J . Anti-Cancer Effects of Asiatic Acid, a Triterpene from Centilla asiatica L: A Review. Anticancer Agents Med Chem. 2019; 20(5):536-547. DOI: 10.2174/1871520619666191211103006. View

Wang Y, Lu C, Zhao X, Wang D, Liu Y, Sun S . Antifungal activity and potential mechanism of Asiatic acid alone and in combination with fluconazole against Candida albicans. Biomed Pharmacother. 2021; 139:111568. DOI: 10.1016/j.biopha.2021.111568. View

10.

Fan C, Yuan Q, Liu G, Zhang Y, Yan M, Sun Q . Long non-coding RNA MALAT1 regulates oxaliplatin-resistance via miR-324-3p/ADAM17 axis in colorectal cancer cells. Cancer Cell Int. 2020; 20:473. PMC: 7525982. DOI: 10.1186/s12935-020-01549-5. View

11.

Khare V, Tabassum S, Chatterjee U, Chatterjee S, Ghosh M . RNA helicase p68 deploys β-catenin in regulating RelA/p65 gene expression: implications in colon cancer. J Exp Clin Cancer Res. 2019; 38(1):330. PMC: 6660689. DOI: 10.1186/s13046-019-1304-y. View

12.

Elumalai P, Ezhilarasan D, Raghunandhakumar S . Quercetin Inhibits the Epithelial to Mesenchymal Transition through Suppressing Akt Mediated Nuclear Translocation of β-Catenin in Lung Cancer Cell Line. Nutr Cancer. 2021; 74(5):1894-1906. DOI: 10.1080/01635581.2021.1957487. View

13.

Zheng X, Ren J, Peng B, Ye J, Wu X, Zhao W . MALAT1 overexpression promotes the growth of colon cancer by repressing β-catenin degradation. Cell Signal. 2020; 73:109676. DOI: 10.1016/j.cellsig.2020.109676. View

14.

Zhang X, Hamblin M, Yin K . The long noncoding RNA Malat1: Its physiological and pathophysiological functions. RNA Biol. 2017; 14(12):1705-1714. PMC: 5731810. DOI: 10.1080/15476286.2017.1358347. View

15.

Wang Y, Wang Y, Qin Z, Cai S, Yu L, Hu H . The role of non-coding RNAs in ABC transporters regulation and their clinical implications of multidrug resistance in cancer. Expert Opin Drug Metab Toxicol. 2021; 17(3):291-306. DOI: 10.1080/17425255.2021.1887139. View

16.

Gutierrez-Salmeron M, Garcia-Martinez J, Martinez-Useros J, Fernandez-Acenero M, Viollet B, Olivier S . Paradoxical activation of AMPK by glucose drives selective EP300 activity in colorectal cancer. PLoS Biol. 2020; 18(6):e3000732. PMC: 7326158. DOI: 10.1371/journal.pbio.3000732. View

17.

Xu J, Chen Z, Fang Z, Chen S, Guo Y, Liu X . Long non-coding RNA OIP5-AS1 promotes the progression of esophageal cancer by regulating miR-30a/VOPP1 expression. Oncol Lett. 2021; 22(3):651. PMC: 8299025. DOI: 10.3892/ol.2021.12912. View

18.

Du B, Wang J, Zang S, Mao X, Du Y . Long non-coding RNA MALAT1 suppresses the proliferation and migration of endothelial progenitor cells in deep vein thrombosis by regulating the Wnt/β-catenin pathway. Exp Ther Med. 2020; 20(4):3138-3146. PMC: 7444359. DOI: 10.3892/etm.2020.9066. View

19.

Huang J, Chen Z, Lai Z, Liu Y, Yu D, Wu L . Kaempferol ameliorates the regulatory effects of / on epithelial-mesenchymal transition through the PAK4/β-catenin axis in SRA01/04 cells. Future Med Chem. 2021; 13(7):613-623. DOI: 10.4155/fmc-2020-0381. View

20.

Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N . Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer. Biochem Biophys Res Commun. 2013; 436(2):319-24. DOI: 10.1016/j.bbrc.2013.05.101. View