Sp. Nov., Isolated from the Gut of Honeybee ()

A novel bifidobacteria (designated S053-2) was isolated from the gut of honeybee (). Strain S053-2 was characterized using a polyphasic taxonomic approach. The result of 16S rRNA gene sequence analysis indicated that strain S053-2 was phylogenetically related to the type strains of , , , , , , and , and had 95.5-99.7 % 16S rRNA gene sequence similarities. Based on the 16S rRNA gene sequence analysis, strain S053-2 was most closely related to the type strain of , having 99.7 % 16S rRNA gene sequence similarity. Strain S053-2 had relatively low (91.6-95.7 %) , , , , , , , , , and sequence similarities to the type strain of . Strain S053-2 had 94.5-95.3% , , , and sequence similarities to the type strain of . The phylogenomic tree indicated that strain S053-2 belonged to the group, and was most closely related to the type strains of , , and , and distantly related to type strains of other phylogenetically related species in the group. Strain S053-2 shared the highest average nucleotide identity (ANI, 93.8 %), digital DNA-DNA hybridization (dDDH, 52.4 %) and average amino acid identity (AAI, 95.6%) values with W8102. Strain S053-2 shared 91.1 % ANI, 41.9 % dDDH and 92.5 % AAI values with DSM 20089. Acid production from l-arabinose, d-xylose, d-mannose, amygdalin, cellobiose, maltose, melibiose, sucrose, raffinose, gentiobiose and l-fucose, and activity of esterase lipase (C8) and -fucosidase could differentiate strain S053-2 from DSM 20089. Acid production from d-mannose, maltose, sucrose, melezitose and gentiobiose, and activity of -fucosidase could differentiate strain S053-2 from W8102. Based upon the data obtained in the present study, a novel species, sp. nov., is proposed, and the type strain is S053-2 (=JCM 34710=CCTCC AB 2021129).