Extract Ameliorates LPS-Induced Septic Shock in Mice

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2022 May 24

PMID 35607518

Authors

Yuan Yee Lee

Heung Joo Yuk

Evelyn Saba

Sung Dae Kim

Dong-Seon Kim

Spandana Rajendra Kopalli

Jae-Wook Oh

Man Hee Rhee

Affiliations

Soon will be listed here.

Abstract

Objective: has been reported for its anti-inflammatory properties. However, its efficacy in sepsis has yet to be reported. In this study, we studied the ability of extract (DIE) to rescue mice from septic shock and sepsis.

Methods: studies included the measurement of secreted nitric oxide, cell viability, gene and protein expression via real-time polymerase chain reaction and western blot, and confocal microscopy in RAW 264.7 cells. studies include a model of septic shock and sepsis in BALB/c mice induced by a lethal and sub-lethal dose of lipopolysaccharide (LPS).

Results: DIE suppressed the expression of proinflammatory cytokines induced by LPS and prevented the translocation of NFB into the nucleus of RAW 264.7 cells. It also prevented reactive oxygen species damage induced by LPS in murine bone marrow-derived macrophages. Models of sepsis and septic shock were established in BALB/ mice and DIE-rescued mice from septic shock. DIE also reversed the increase in tumor necrosis factor- and nitrite levels in the serum of mice induced with sepsis. DIE also prevented the translocation of NFB from the cytosol into the nucleus in murine lungs. Histopathological damage induced by sepsis was reversed in the testis, liver, and lungs of mice.

Conclusion: In conclusion, DIE is a suitable candidate for development as a therapeutic agent for sepsis.

Citing Articles

Biosynthesis of Silver Nanoparticles from Extracts Using Different Solvents and Their Antibacterial Activity.

Kim S, Choi H, Lim J, Woo M, Chang H, Lee N Microorganisms. 2023; 11(6).

PMID: 37375043 PMC: 10301177. DOI: 10.3390/microorganisms11061539.

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