Urine 1H-NMR Metabolomics to Discriminate Neurocysticercosis Patients from Healthy Controls: An Exploratory Study

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2022 May 23

PMID 35605633

Authors

Juan M Lopez

Vanessa E Leyva

Javier A Bustos

Erika Perez

Sofia Sanchez

Herbert Saavedra

Isidro Gonzales

Helena Maruenda

Hector H Garcia

Affiliations

Soon will be listed here.

Abstract

The diagnosis of neurocysticercosis (NCC) is principally based on neuroimaging (magnetic resonance imaging or computed tomography), instrumentation that is scarcely available in the rural regions where Taenia solium transmission, primarily occurs due to poor sanitation conditions. Immunological assays for antigen or antibody detection complement the neuroimaging approach. However, no field-applicable assays to diagnose viable NCC or to guide the referral of cases for neuroimaging or for appropriate management are available. We performed an exploratory study on urine and serum samples using 1H-nuclear magnetic resonance (NMR)-based metabolomics to discriminate NCC patients (n = 14) from healthy control subjects (n = 22). Metabolic profiles demonstrated a discrimination between the urines of NCC patients and noninfected control subjects with a moderate predictive accuracy (R2 = 0.999, Q2 = 0.434). NMR metabolomics analysis has been proven useful in depicting biomarkers linked to other infectious diseases, various types of cancer, and other disorders. Our results, albeit preliminary, open a door to the development of better methods for detecting NCC through the identification of biomarkers participating in disturbed metabolic pathways.

References

DeFeo E, Wu C, McDougal W, Cheng L . A decade in prostate cancer: from NMR to metabolomics. Nat Rev Urol. 2011; 8(6):301-11. DOI: 10.1038/nrurol.2011.53. View

Sinclair A, Viant M, Ball A, Burdon M, Walker E, Stewart P . NMR-based metabolomic analysis of cerebrospinal fluid and serum in neurological diseases--a diagnostic tool?. NMR Biomed. 2009; 23(2):123-32. DOI: 10.1002/nbm.1428. View

Slupsky C . NMR-based analysis of metabolites in urine provides rapid diagnosis and etiology of pneumonia. Biomark Med. 2010; 4(2):195-7. DOI: 10.2217/bmm.10.11. View

Castillo Y, Rodriguez S, Garcia H, Brandt J, Van Hul A, Silva M . Urine antigen detection for the diagnosis of human neurocysticercosis. Am J Trop Med Hyg. 2009; 80(3):379-83. View

Ahmed S, Santosh W, Kumar S, Christlet H . Metabolic profiling of Parkinson's disease: evidence of biomarker from gene expression analysis and rapid neural network detection. J Biomed Sci. 2009; 16:63. PMC: 2720938. DOI: 10.1186/1423-0127-16-63. View

Beckonert O, Keun H, Ebbels T, Bundy J, Holmes E, Lindon J . Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts. Nat Protoc. 2007; 2(11):2692-703. DOI: 10.1038/nprot.2007.376. View

Gebregiworgis T, Powers R . Application of NMR metabolomics to search for human disease biomarkers. Comb Chem High Throughput Screen. 2012; 15(8):595-610. PMC: 6625354. DOI: 10.2174/138620712802650522. View

Garcia H, Rodriguez S, Gilman R, Gonzalez A, Tsang V . Neurocysticercosis: is serology useful in the absence of brain imaging?. Trop Med Int Health. 2012; 17(8):1014-8. DOI: 10.1111/j.1365-3156.2012.03037.x. View

Shin J, Yang J, Jeon B, Yoon Y, Cho S, Kang Y . (1)H NMR-based metabolomic profiling in mice infected with Mycobacterium tuberculosis. J Proteome Res. 2011; 10(5):2238-47. DOI: 10.1021/pr101054m. View

10.

Toribio L, Romano M, Scott A, Gonzales I, Saavedra H, Garcia H . Detection of DNA in the Urine of Neurocysticercosis Patients. Am J Trop Med Hyg. 2018; 100(2):327-329. PMC: 6367601. DOI: 10.4269/ajtmh.18-0706. View

11.

Toribio L, Santivanez S, Scott A, Enriquez R, Sedano C, Soto-Becerra P . Diagnostic urinary cfDNA detected in human cystic echinococcosis. Mol Biochem Parasitol. 2020; 239:111314. PMC: 8801304. DOI: 10.1016/j.molbiopara.2020.111314. View

12.

Ranjan R, Sinha N . Nuclear magnetic resonance (NMR)-based metabolomics for cancer research. NMR Biomed. 2018; 32(10):e3916. DOI: 10.1002/nbm.3916. View

13.

Aguilar J, Nilsson M, Bodenhausen G, Morris G . Spin echo NMR spectra without J modulation. Chem Commun (Camb). 2011; 48(6):811-3. DOI: 10.1039/c1cc16699a. View

14.

Garcia H, Gonzalez A, Gilman R . Taenia solium Cysticercosis and Its Impact in Neurological Disease. Clin Microbiol Rev. 2020; 33(3). PMC: 7254859. DOI: 10.1128/CMR.00085-19. View

15.

McCleery E, Allen S, Moyano L, Gamboa R, Vilchez P, Muro C . Population Screening for Urine Antigens to Detect Asymptomatic Subarachnoid Neurocysticercosis: A Pilot Study in Northern Peru. Am J Trop Med Hyg. 2020; 103(3):1125-1128. PMC: 7470589. DOI: 10.4269/ajtmh.20-0196. View

16.

Teng R, Junankar P, Bubb W, Rae C, Mercier P, Kirk K . Metabolite profiling of the intraerythrocytic malaria parasite Plasmodium falciparum by (1)H NMR spectroscopy. NMR Biomed. 2008; 22(3):292-302. DOI: 10.1002/nbm.1323. View

17.

Del Brutto O, Nash T, White Jr A, Rajshekhar V, Wilkins P, Singh G . Revised diagnostic criteria for neurocysticercosis. J Neurol Sci. 2016; 372:202-210. DOI: 10.1016/j.jns.2016.11.045. View

18.

Beger R . A review of applications of metabolomics in cancer. Metabolites. 2014; 3(3):552-74. PMC: 3901293. DOI: 10.3390/metabo3030552. View