Mouse Splenocyte Enrichment Strategies Via Negative Selection for Broadened Single-cell Transcriptomics

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2022 May 23

PMID 35600930

Authors

Thomas T Schulze

Andrew J Neville

Ryan C Chapman

Paul H Davis

Affiliations

Soon will be listed here.

Abstract

Mammalian splenic tissue is rich in functional immune cells, primarily lymphocytes which can mask low-abundance populations in downstream analyses. This protocol enriches minority immune cell populations from mouse spleen via immunomagnetic negative depletion to generate an untouched enriched cell fraction. Enriched cells are then spiked with untouched splenocytes in a controlled repopulation, validated by flow cytometry and results in a single-cell transcriptomic clustering analysis with a broadened cellular landscape.

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