A Protocol to Evaluate Immunoglobulin Deposits in Mouse Glomeruli

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2022 May 23

PMID 35600919

Authors

Sonia B Nambadan

Maurice Stanley

Yaoyuan Zhang

Vicki Athanasopoulos

Simon H Jiang

Affiliations

Soon will be listed here.

Abstract

Accumulation of immunoglobulin and complement components within the kidneys is a hallmark of glomerulonephritis. Staining and detection of IgG, IgA, IgM, and C3 deposits can assist in diagnosing the underlying causes of nephritis and has implications for the pathological processes underpinning glomerulonephritis. Here, we describe a protocol to detect immune deposits within biological specimens such as mouse kidneys. We detail tissue isolation and processing, immunostaining, and fluorescence microscopy to characterize and quantify the extent of immunological deposits contributing to kidney injury. For complete details on the use and execution of this protocol, please refer to Jiang et al. (2021).

Citing Articles

Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy.

Lopez-Tello J, Jimenez-Martinez M, Salazar-Petres E, Patel R, George A, Kay R Int J Mol Sci. 2022; 23(11).

PMID: 35682969 PMC: 9181623. DOI: 10.3390/ijms23116287.

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