LINC01806 Mediated by STAT1 Promotes Cell Proliferation, Migration, Invasion, and Stemness in Non-small Cell Lung Cancer Through Notch Signaling by MiR-4428/NOTCH2 Axis

Overview

Journal Cancer Cell Int

Publisher Biomed Central

Date 2022 May 22

PMID 35599309

Authors

Shangxiao Huang

Shixiong Liang

Jianfeng Huang

Penghui Luo

Dunchang Mo

Hanlei Wang

Affiliations

Soon will be listed here.

Abstract

Background: Non-small cell lung cancer (NSCLC), the most primary lung cancer subtype, threatens human health globally. Long non-coding RNAs (lncRNAs) have been uncovered to affect multiple cancers progression. Nevertheless, the specific function of long intergenic non-protein coding RNA 1806 (LINC01806) in NSCLC remains elusive.

Methods: RT-qPCR and western blot were involved in this study. The influence of LINC01806 on NSCLC was assessed by in vitro and in vivo assays. Via ChIP, RNA pull down, RIP, and luciferase reporter assays, the in-depth cellular mechanisms of LINC01806 in NSCLC were explored.

Results: LINC01806 expression was high in NSCLC cell lines. Functionally, LINC01806 knockdown impeded cell proliferation, migration, invasion, and stemness, along with tumor growth. As for its mechanism, signal transducer and activator of transcription 1 (STAT1) activated LINC01806 transcription in NSCLC. Furthermore, LINC01806 sequestered microRNA-4428 (miR-4428) to enhance notch receptor 2 (NOTCH2) expression, thus activating Notch signaling pathway. Finally, in vitro and in vivo assays jointly validated that LINC01806 exerted its function in NSCLC development via miR-4428/NOTCH2 pathway.

Conclusion: LINC01806 enhanced NOTCH2 expression to stimulate Notch signaling via sponging miR-4428, thereby facilitating NSCLC progression, which provided a novel mechanism for NSCLC therapeutic approaches.

Citing Articles

Interplay between JAK/STAT pathway and non-coding RNAs in different cancers.

Farooqi A, Shepetov A, Rakhmetova V, Ruslan Z, Almabayeva A, Saussakova S Noncoding RNA Res. 2024; 9(4):1009-1022.

PMID: 39022684 PMC: 11254501. DOI: 10.1016/j.ncrna.2024.04.001.

LINC01806 Promotes Breast Cancer Growth and Metastasis via Sponging miR-1286 to Disinhibit ZEB1 Expression.

Liu Y, Xiang Q, Yang T, Wang J, Li H Biochem Genet. 2023; 62(3):1977-1993.

PMID: 37812283 DOI: 10.1007/s10528-023-10507-5.

Upregulated SPAG6 correlates with increased STAT1 and is associated with reduced sensitivity of interferon-α response in BCR::ABL1 negative myeloproliferative neoplasms.

Ding L, Luo J, Du J, Zhao B, Luo J, Pan S Cancer Sci. 2023; 114(11):4445-4458.

PMID: 37681349 PMC: 10637088. DOI: 10.1111/cas.15950.

DDX18 drives tumor immune escape through transcription-activated STAT1 expression in pancreatic cancer.

Dong G, Wang Q, Wen M, Xia Z, Zhang S, Gao W Oncogene. 2023; 42(40):3000-3014.

PMID: 37620449 DOI: 10.1038/s41388-023-02817-0.

Emerging roles of PHLPP phosphatases in lung cancer.

Xia X, Pi W, Chen M, Wang W, Cai D, Wang X Front Oncol. 2023; 13:1216131.

PMID: 37576883 PMC: 10414793. DOI: 10.3389/fonc.2023.1216131.

References

Fuchs J, Fruh M, Papachristofilou A, Bubendorf L, Hauptle P, Jost L . Resection of isolated brain metastases in non-small cell lung cancer (NSCLC) patients - evaluation of outcome and prognostic factors: A retrospective multicenter study. PLoS One. 2021; 16(6):e0253601. PMC: 8238224. DOI: 10.1371/journal.pone.0253601. View

Pennell N, Arcila M, Gandara D, West H . Biomarker Testing for Patients With Advanced Non-Small Cell Lung Cancer: Real-World Issues and Tough Choices. Am Soc Clin Oncol Educ Book. 2019; 39:531-542. DOI: 10.1200/EDBK_237863. View

Shi J, Li J, Yang S, Hu X, Chen J, Feng J . LncRNA SNHG3 is activated by E2F1 and promotes proliferation and migration of non-small-cell lung cancer cells through activating TGF-β pathway and IL-6/JAK2/STAT3 pathway. J Cell Physiol. 2019; 235(3):2891-2900. DOI: 10.1002/jcp.29194. View

Li J, Meng H, Bai Y, Wang K . Regulation of lncRNA and Its Role in Cancer Metastasis. Oncol Res. 2016; 23(5):205-17. PMC: 7838649. DOI: 10.3727/096504016X14549667334007. View

Dandan W, Jianliang C, Haiyan H, Hang M, Xuedong L . Long noncoding RNA MIR31HG is activated by SP1 and promotes cell migration and invasion by sponging miR-214 in NSCLC. Gene. 2019; 692:223-230. DOI: 10.1016/j.gene.2018.12.077. View

Li L, Tang P, Li S, Qin X, Yang H, Wu C . Notch signaling pathway networks in cancer metastasis: a new target for cancer therapy. Med Oncol. 2017; 34(10):180. DOI: 10.1007/s12032-017-1039-6. View

Wang X, Yin H, Zhang L, Zheng D, Yang Y, Zhang J . The construction and analysis of the aberrant lncRNA-miRNA-mRNA network in non-small cell lung cancer. J Thorac Dis. 2019; 11(5):1772-1778. PMC: 6588744. DOI: 10.21037/jtd.2019.05.69. View

Chen S, Zhu J, Wang F, Guan Z, Ge Y, Yang X . LncRNAs and their role in cancer stem cells. Oncotarget. 2018; 8(66):110685-110692. PMC: 5746414. DOI: 10.18632/oncotarget.22161. View

Zhang K, Hong X, Song Z, Xu Y, Li C, Wang G . Identification of Deleterious Mutation as Novel Predictor to Efficacious Immunotherapy in NSCLC. Clin Cancer Res. 2020; 26(14):3649-3661. DOI: 10.1158/1078-0432.CCR-19-3976. View

10.

Prasad S, Ramachandran S, Gupta N, Kaushik I, Srivastava S . Cancer cells stemness: A doorstep to targeted therapy. Biochim Biophys Acta Mol Basis Dis. 2019; 1866(4):165424. DOI: 10.1016/j.bbadis.2019.02.019. View

11.

Yang J, Li C, Mudd A, Gu X . LncRNA PVT1 predicts prognosis and regulates tumor growth in prostate cancer. Biosci Biotechnol Biochem. 2017; 81(12):2301-2306. DOI: 10.1080/09168451.2017.1387048. View

12.

Walker S . Updates in non-small cell lung cancer. Clin J Oncol Nurs. 2008; 12(4):587-96. DOI: 10.1188/08.CJON.587-596. View

13.

Meng S, Jian Z, Yan X, Li J, Zhang R . LncRNA SNHG6 inhibits cell proliferation and metastasis by targeting ETS1 via the PI3K/AKT/mTOR pathway in colorectal cancer. Mol Med Rep. 2019; 20(3):2541-2548. PMC: 6691244. DOI: 10.3892/mmr.2019.10510. View

14.

Jia Y, Wang J, Liu Y, Li B, Guo H, Zang A . LncRNA MAFG-AS1 facilitates the migration and invasion of NSCLC cell via sponging miR-339-5p from MMP15. Cell Biol Int. 2019; 43(4):384-393. DOI: 10.1002/cbin.11092. View

15.

Qu Y, Cheng B, Shao N, Jia Y, Song Q, Tan B . Prognostic value of immune-related genes in the tumor microenvironment of lung adenocarcinoma and lung squamous cell carcinoma. Aging (Albany NY). 2020; 12(6):4757-4777. PMC: 7138544. DOI: 10.18632/aging.102871. View

16.

Cui Y, Zhang F, Zhu C, Geng L, Tian T, Liu H . Upregulated lncRNA SNHG1 contributes to progression of non-small cell lung cancer through inhibition of miR-101-3p and activation of Wnt/β-catenin signaling pathway. Oncotarget. 2017; 8(11):17785-17794. PMC: 5392286. DOI: 10.18632/oncotarget.14854. View

17.

Braune E, Lendahl U . Notch -- a goldilocks signaling pathway in disease and cancer therapy. Discov Med. 2016; 21(115):189-96. View

18.

Zou B, Zhou X, Lai S, Liu J . Notch signaling and non-small cell lung cancer. Oncol Lett. 2018; 15(3):3415-3421. PMC: 5796339. DOI: 10.3892/ol.2018.7738. View

19.

Du W, Feng Z, Sun Q . LncRNA LINC00319 accelerates ovarian cancer progression through miR-423-5p/NACC1 pathway. Biochem Biophys Res Commun. 2018; 507(1-4):198-202. DOI: 10.1016/j.bbrc.2018.11.006. View

20.

Chan J, Tay Y . Noncoding RNA:RNA Regulatory Networks in Cancer. Int J Mol Sci. 2018; 19(5). PMC: 5983611. DOI: 10.3390/ijms19051310. View