Characterization of Extra-Cellular Vesicle Dielectrophoresis and Estimation of Its Electric Properties

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2022 May 20

PMID 35590969

Authors

Hao Chen

Tsubasa Yamakawa

Masafumi Inaba

Michihiko Nakano

Junya Suehiro

Affiliations

Soon will be listed here.

Abstract

Dielectrophoresis (DEP) refers to a type of electrical motion of dielectric particles. Because DEP is caused by particle polarization, it has been utilized to characterize particles. This study investigated the DEP of three types of exosomes, namely bovine milk, human breast milk, and human breast cancer exosomes. Exosomes are kinds of extracellular vesicles. The crossover frequencies of the exosomes were determined by direct observation of their DEPs. Consequently, bovine and human milk exosomes showed similar DEP properties, whereas the cancer exosomes were significantly different from the others. The membrane capacitance and conductivity of the exosomes were estimated using determined values. A significant difference was observed between bovine and human milk exosomes on their membrane capacitance. It was revealed that the membrane capacitances of human breast milk and human breast cancer exosomes were almost identical to those of their host cells and the conductivity of the exosomes were much lower than that of the host cell. Based on these results, DEP separation of the human breast milk and cancer exosomes was demonstrated. These results imply that DEP can be utilized to separate and identify cancer exosomes rapidly. Additionally, our method can be utilized to estimate the electric property of other types of extracellular vesicles.

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References

Zhang Y, Liu Y, Liu H, Tang W . Exosomes: biogenesis, biologic function and clinical potential. Cell Biosci. 2019; 9:19. PMC: 6377728. DOI: 10.1186/s13578-019-0282-2. View

Shimomura T, Seino R, Umezaki K, Shimoda A, Ezoe T, Ishiyama M . New Lipophilic Fluorescent Dyes for Labeling Extracellular Vesicles: Characterization and Monitoring of Cellular Uptake. Bioconjug Chem. 2021; 32(4):680-684. DOI: 10.1021/acs.bioconjchem.1c00068. View

Chan J, Ahmad Kayani A, Md Ali M, Kok C, Yeop Majlis B, Hoe S . Dielectrophoresis-based microfluidic platforms for cancer diagnostics. Biomicrofluidics. 2018; 12(1):011503. PMC: 5825230. DOI: 10.1063/1.5010158. View

Gagnon Z . Cellular dielectrophoresis: applications to the characterization, manipulation, separation and patterning of cells. Electrophoresis. 2011; 32(18):2466-87. DOI: 10.1002/elps.201100060. View

Emelyanov A, Shtam T, Kamyshinsky R, Garaeva L, Verlov N, Miliukhina I . Cryo-electron microscopy of extracellular vesicles from cerebrospinal fluid. PLoS One. 2020; 15(1):e0227949. PMC: 6991974. DOI: 10.1371/journal.pone.0227949. View

Ermolina I, Milner J, Morgan H . Dielectrophoretic investigation of plant virus particles: Cow Pea Mosaic Virus and Tobacco Mosaic Virus. Electrophoresis. 2006; 27(20):3939-48. DOI: 10.1002/elps.200500928. View

Ermolina I, Morgan H . The electrokinetic properties of latex particles: comparison of electrophoresis and dielectrophoresis. J Colloid Interface Sci. 2005; 285(1):419-28. DOI: 10.1016/j.jcis.2004.11.003. View

Hughes M, Morgan H, Rixon F . Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis. Biochim Biophys Acta. 2002; 1571(1):1-8. DOI: 10.1016/s0304-4165(02)00161-7. View

Shao H, Im H, Castro C, Breakefield X, Weissleder R, Lee H . New Technologies for Analysis of Extracellular Vesicles. Chem Rev. 2018; 118(4):1917-1950. PMC: 6029891. DOI: 10.1021/acs.chemrev.7b00534. View

10.

Turcan I, Olariu M . Dielectrophoretic Manipulation of Cancer Cells and Their Electrical Characterization. ACS Comb Sci. 2020; 22(11):554-578. DOI: 10.1021/acscombsci.0c00109. View

11.

Coley H, Labeed F, Thomas H, Hughes M . Biophysical characterization of MDR breast cancer cell lines reveals the cytoplasm is critical in determining drug sensitivity. Biochim Biophys Acta. 2007; 1770(4):601-8. DOI: 10.1016/j.bbagen.2006.12.002. View

12.

Shim S, Stemke-Hale K, Noshari J, Becker F, Gascoyne P . Dielectrophoresis has broad applicability to marker-free isolation of tumor cells from blood by microfluidic systems. Biomicrofluidics. 2014; 7(1):11808. PMC: 3562275. DOI: 10.1063/1.4774307. View

13.

Diaz-Armas G, Cervantes-Gonzalez A, Martinez-Duarte R, Perez-Gonzalez V . Electrically driven microfluidic platforms for exosome manipulation and characterization. Electrophoresis. 2021; 43(1-2):327-339. DOI: 10.1002/elps.202100202. View

14.

Frusawa H . Frequency-Modulated Wave Dielectrophoresis of Vesicles And Cells: Periodic U-Turns at the Crossover Frequency. Nanoscale Res Lett. 2018; 13(1):169. PMC: 5991112. DOI: 10.1186/s11671-018-2583-5. View

15.

van Niel G, DAngelo G, Raposo G . Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018; 19(4):213-228. DOI: 10.1038/nrm.2017.125. View

16.

Ayala-Mar S, Perez-Gonzalez V, Mata-Gomez M, Gallo-Villanueva R, Gonzalez-Valdez J . Electrokinetically Driven Exosome Separation and Concentration Using Dielectrophoretic-Enhanced PDMS-Based Microfluidics. Anal Chem. 2019; 91(23):14975-14982. DOI: 10.1021/acs.analchem.9b03448. View

17.

Pohl H, Hawk I . Separation of living and dead cells by dielectrophoresis. Science. 1966; 152(3722):647-9. DOI: 10.1126/science.152.3722.647-a. View

18.

Moura S, Marti M, Pividori M . Matrix Effect in the Isolation of Breast Cancer-Derived Nanovesicles by Immunomagnetic Separation and Electrochemical Immunosensing-A Comparative Study. Sensors (Basel). 2020; 20(4). PMC: 7071381. DOI: 10.3390/s20040965. View

19.

Shim S, Gascoyne P, Noshari J, Hale K . Dynamic physical properties of dissociated tumor cells revealed by dielectrophoretic field-flow fractionation. Integr Biol (Camb). 2011; 3(8):850-62. PMC: 3864024. DOI: 10.1039/c1ib00032b. View

20.

Lei U, Sun P, Pethig R . Refinement of the theory for extracting cell dielectric properties from dielectrophoresis and electrorotation experiments. Biomicrofluidics. 2012; 5(4):44109-4410916. PMC: 3364808. DOI: 10.1063/1.3659282. View