Ubiquitin Ligase OsRINGzf1 Regulates Drought Resistance by Controlling the Turnover of OsPIP2;1

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2022 May 19

PMID 35587579

Authors

Shoujun Chen

Kai Xu

Deyan Kong

Lunying Wu

Qian Chen

Xiaosong Ma

Siqi Ma

Tianfei Li

Qi Xie

Hongyan Liu

Lijun Luo

Affiliations

Soon will be listed here.

Abstract

Water is crucial for plant growth and survival. The transcellular water movement is facilitated by aquaporins (AQPs) that rapidly and reversibly modify water permeability. The abundance of AQPs is regulated by its synthesis, redistribution and degradation. However, the molecular mechanism of proteasomal degradation of AQPs remains unclear. Here, we demonstrate that a novel E3 ligase, OsRINGzf1, mediated the degradation of AQPs in rice. OsRINGzf1 is the candidate gene from a drought-related quantitative trait locus (QTL) on the long arm of chromosome 4 in rice (Oryza sativa) and encodes a Really Interesting New Gene (RING) zinc finger protein 1. OsRINGzf1 possesses the E3 ligase activity, ubiquitinates and mediates OsPIP2;1 degradation, thus reducing its protein abundance. The content of OsPIP2;1 protein was decreased in OsRINGzf1 overexpression (OE) plants. The degradation of OsPIP2;1 was inhibited by MG132. The OsRINGzf1 OE plants, with higher leaf-related water content (LRWC) and lower leaf water loss rate (LWLR), exhibited enhanced drought resistance, whereas the RNAi and knockout plants of OsRINGzf1 were more sensitive to drought. Together, our data demonstrate that OsRINGzf1 positively regulates drought resistance through promoting the degradation of OsPIP2;1 to enhance water retention capacity in rice.

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References

Bai J, Wang X, Yao X, Chen X, Lu K, Hu Y . Rice aquaporin OsPIP2;2 is a water-transporting facilitator in relevance to drought-tolerant responses. Plant Direct. 2021; 5(8):e338. PMC: 8365552. DOI: 10.1002/pld3.338. View

Xie Q, Guo H, Dallman G, Fang S, Weissman A, Chua N . SINAT5 promotes ubiquitin-related degradation of NAC1 to attenuate auxin signals. Nature. 2002; 419(6903):167-70. DOI: 10.1038/nature00998. View

Matsumoto T, Lian H, Su W, Tanaka D, Liu C, Iwasaki I . Role of the aquaporin PIP1 subfamily in the chilling tolerance of rice. Plant Cell Physiol. 2008; 50(2):216-29. DOI: 10.1093/pcp/pcn190. View

Kim J, Jang C . E3 ligase, the Oryza sativa salt-induced RING finger protein 4 (OsSIRP4), negatively regulates salt stress responses via degradation of the OsPEX11-1 protein. Plant Mol Biol. 2020; 105(3):231-245. DOI: 10.1007/s11103-020-01084-x. View

Takano J, Wada M, Ludewig U, Schaaf G, von Wiren N, Fujiwara T . The Arabidopsis major intrinsic protein NIP5;1 is essential for efficient boron uptake and plant development under boron limitation. Plant Cell. 2006; 18(6):1498-509. PMC: 1475503. DOI: 10.1105/tpc.106.041640. View

Biela A, Grote K, Otto B, Hoth S, Hedrich R, Kaldenhoff R . The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol. Plant J. 1999; 18(5):565-70. DOI: 10.1046/j.1365-313x.1999.00474.x. View

Martre P, Morillon R, Barrieu F, North G, Nobel P, Chrispeels M . Plasma membrane aquaporins play a significant role during recovery from water deficit. Plant Physiol. 2002; 130(4):2101-10. PMC: 166722. DOI: 10.1104/pp.009019. View

Park Y, Chapagain S, Jang C . A Negative Regulator in Response to Salinity in Rice: Oryza sativa Salt-, ABA- and Drought-Induced RING Finger Protein 1 (OsSADR1). Plant Cell Physiol. 2018; 59(3):575-589. DOI: 10.1093/pcp/pcy009. View

Chapagain S, Park Y, Kim J, Jang C . Oryza sativa salt-induced RING E3 ligase 2 (OsSIRP2) acts as a positive regulator of transketolase in plant response to salinity and osmotic stress. Planta. 2017; 247(4):925-939. DOI: 10.1007/s00425-017-2838-x. View

10.

Park Y, Lim S, Moon J, Jang C . A rice really interesting new gene H2-type E3 ligase, OsSIRH2-14, enhances salinity tolerance via ubiquitin/26S proteasome-mediated degradation of salt-related proteins. Plant Cell Environ. 2019; 42(11):3061-3076. DOI: 10.1111/pce.13619. View

11.

Fox A, Maistriaux L, Chaumont F . Toward understanding of the high number of plant aquaporin isoforms and multiple regulation mechanisms. Plant Sci. 2017; 264:179-187. DOI: 10.1016/j.plantsci.2017.07.021. View

12.

Laloux T, Junqueira B, Maistriaux L, Ahmed J, Jurkiewicz A, Chaumont F . Plant and Mammal Aquaporins: Same but Different. Int J Mol Sci. 2018; 19(2). PMC: 5855743. DOI: 10.3390/ijms19020521. View

13.

Zargar S, Nagar P, Deshmukh R, Nazir M, Wani A, Masoodi K . Aquaporins as potential drought tolerance inducing proteins: Towards instigating stress tolerance. J Proteomics. 2017; 169:233-238. DOI: 10.1016/j.jprot.2017.04.010. View

14.

Luo L . Breeding for water-saving and drought-resistance rice (WDR) in China. J Exp Bot. 2010; 61(13):3509-17. DOI: 10.1093/jxb/erq185. View

15.

Lian H, Yu X, Ye Q, Ding X, Kitagawa Y, Kwak S . The role of aquaporin RWC3 in drought avoidance in rice. Plant Cell Physiol. 2004; 45(4):481-9. DOI: 10.1093/pcp/pch058. View

16.

Hove R, Bhave M . Plant aquaporins with non-aqua functions: deciphering the signature sequences. Plant Mol Biol. 2011; 75(4-5):413-30. DOI: 10.1007/s11103-011-9737-5. View

17.

Chen Q, Liu R, Wu Y, Wei S, Wang Q, Zheng Y . ERAD-related E2 and E3 enzymes modulate the drought response by regulating the stability of PIP2 aquaporins. Plant Cell. 2021; 33(8):2883-2898. PMC: 8408458. DOI: 10.1093/plcell/koab141. View

18.

Park Y, Chapagain S, Jang C . The microtubule-associated RING finger protein 1 (OsMAR1) acts as a negative regulator for salt-stress response through the regulation of OCPI2 (O. sativa chymotrypsin protease inhibitor 2). Planta. 2017; 247(4):875-886. DOI: 10.1007/s00425-017-2834-1. View

19.

Li X, Liu Q, Feng H, Deng J, Zhang R, Wen J . Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy. 2019; 16(5):862-877. PMC: 7144882. DOI: 10.1080/15548627.2019.1643656. View

20.

Noronha H, Agasse A, Martins A, Berny M, Gomes D, Zarrouk O . The grape aquaporin VvSIP1 transports water across the ER membrane. J Exp Bot. 2013; 65(4):981-93. DOI: 10.1093/jxb/ert448. View