Effective-Component Compatibility of Bufei Yishen Formula III Combined with Electroacupuncture Suppresses Inflammatory Response in Rats with Chronic Obstructive Pulmonary Disease Via Regulating SIRT1/NF-B Signaling

Objective: To explore more efficient treatments for chronic obstructive pulmonary disease (COPD), effective-component compatibility of Bufei Yishen formula III (ECC-BYF III) and electroacupuncture were tested on rats with COPD, and silent information regulator transcript-1 (SIRT1)/nuclear factor-kappaB (NF-B) signaling was further investigated to interpret the therapy.

Methods: In total, 70 rats were randomly divided into control (Control), model (Model), aminophylline (APL), ECC-BYF III, electroacupuncture (EA), ECC-BYF III+EA, and sham electroacupuncture (SA) groups. Cigarette smoke exposure combined with repeated bacterial infections was used to establish COPD models in 1-12 weeks. From 13 to 20 weeks, the ECC-BYF III and APL groups received corresponding drugs; the EA group received electroacupuncture therapy, wherein Dazhui (GV 14), Feishu (BL 13), and Shenshu (BL 23) points were selected; the ECC-BYF III+EA group received ECC-BYF III intragastrically combined with electroacupuncture; and the SA group received simulated electroacupuncture (nonacupoint). Pulmonary function, pulmonary histopathology, the expressions of SIRT1/NF-B signaling, and inflammation-related mRNA and protein were detected.

Results: Significant deterioration was observed in pulmonary function and pulmonary histopathology in rats with COPD ( < 0.01), and inflammatory state was illustrated by increased levels of interleukin- (IL-) 6 and tumor necrosis factor alpha (TNF-) and decreased levels of IL-10 ( < 0.01). After the intervention of APL, ECC-BYF III, EA, and ECC-BYF III+EA, both pulmonary function and pulmonary histopathology were improved ( < 0.05 and < 0.01), whereas the levels of IL-6 and TNF- were decreased and IL-10 was increased ( < 0.05 and < 0.01). Additionally, the mRNA expressions of IL-6, TNF-, NF-B, and acetylated NF-Bp65 (Ac-NF-B) were noted to decrease, and SIRT1 and IL-10 were increased ( < 0.05 and < 0.01); the protein expression of SIRT1 was upregulated, and NF-Bp65 and Ac-NF-B were downregulated ( < 0.05 and < 0.01). The effect of ECC-BYF III+EA was better in terms of improving pulmonary function and alleviating inflammation than that of the other treatment groups ( < 0.01 and < 0.05).

Conclusions: ECC-BYF III, electroacupuncture, and their combination can suppress inflammation, among which the combination therapy has been proven to be the most effective treatment, and the mechanism may be involved in activating SIRT1/NF-B signaling.