Zengye Decoction Attenuated Severe Acute Pancreatitis Complicated with Acute Kidney Injury by Modulating the Gut Microbiome and Serum Amino Acid Metabolome

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2022 May 19

PMID 35586694

Authors

Xiao-Yu Dai

Qian Hu

Jia-Qi Yao

Xiao-Jia Wu

Yi-Fan Miao

Juan Li

Mei-Hua Wan

Wen-Fu Tang

Affiliations

Soon will be listed here.

Abstract

Objective: To explore the effect and underlying mechanism of Zengye decoction (ZYD), a traditional formula from China, on the severe acute pancreatitis (SAP) rat model with acute kidney injury (AKI).

Methods: The SAP-AKI model was induced by 3.5% sodium taurocholate. Rats were treated with normal saline or ZYD twice and sacrificed at 36 h after modeling. Amylase, lipase, creatinine, blood urea nitrogen, kidney injury molecule 1(KIM-1), and multiple organs' pathological examinations were used to assess the protective effect of ZYD. Gut microbiome detected by 16S rRNA sequencing analysis and serum amino acid metabolome analyzed by liquid chromatography-mass spectrometry explained the underlying mechanism. The Spearman correlation analysis presented the relationship between microflora and metabolites.

Results: ZYD significantly decreased KIM-1( < 0.05) and the pathological score of the pancreas ( < 0.05), colon ( < 0.05), and kidney ( < 0.05). Meanwhile, ZYD shifted the overall gut microbial structure (-diversity, ANOSIM = 0.14, =0.025) and altered the microbial compositions. Notably, ZYD reduced the potentially pathogenic bacteria-Bacteroidetes, Clostridiales vadin BB60 group, and uncultured_Clostridiales_bacterium, but promoted the short-chain fatty acid (SCFA) producers-Erysipelotrichaceae, Bifidobacterium, Lactobacillus, and (all < 0.05). Moreover, principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and hierarchical clustering analysis (HCA) presented a remarkable change in amino acid metabolome after SAP-AKI induction and an apparent regulation by ZYD treatment (R2Y 0.878, =0.01; Q2 0.531, =0.01). Spearman's correlation analysis suggested that gut bacteria likely influenced serum metabolites levels (absolute > 0.4 and FDR < 0.02).

Conclusions: ZYD attenuated SAP-AKI by modulating the gut microbiome and serum amino acid metabolome, which may be a promising adjuvant treatment.

Citing Articles

Alterations in microbiome associated with acute pancreatitis.

Yazici C, Priyadarshini M, Boulay B, Dai Y, Layden B Curr Opin Gastroenterol. 2024; 40(5):413-421.

PMID: 38900442 PMC: 11305980. DOI: 10.1097/MOG.0000000000001046.

Investigation into actions of Xiebai and Zengye decoction on cough sensitivity, airway inflammation and gut microbiota in the rat model of post-infectious cough.

Luo J, Deng Y, Ding Y, Zhang Y, Tan M, Xu G Heliyon. 2023; 9(12):e22782.

PMID: 38094068 PMC: 10716551. DOI: 10.1016/j.heliyon.2023.e22782.

References

Feng T, Ding H, Wang J, Xu W, Liu Y, Kenez A . Alterations of Serum Metabolites and Fecal Microbiota Involved in Ewe Follicular Cyst. Front Microbiol. 2021; 12:675480. PMC: 8149755. DOI: 10.3389/fmicb.2021.675480. View

Pan X, Fang X, Wang F, Li H, Niu W, Liang W . Butyrate ameliorates caerulein-induced acute pancreatitis and associated intestinal injury by tissue-specific mechanisms. Br J Pharmacol. 2019; 176(23):4446-4461. PMC: 6932943. DOI: 10.1111/bph.14806. View

Takauji S, Konishi H, Fujiya M, Ueno N, Tanaka H, Sato H . Polyphosphate, Derived from Lactobacillus brevis, Modulates the Intestinal Microbiome and Attenuates Acute Pancreatitis. Dig Dis Sci. 2021; 66(11):3872-3884. DOI: 10.1007/s10620-020-06747-9. View

Rabb H, Pluznick J, Noel S . The Microbiome and Acute Kidney Injury. Nephron. 2018; 140(2):120-123. PMC: 6292672. DOI: 10.1159/000490392. View

Takis P, Taddei A, Pini R, Grifoni S, Tarantini F, Bechi P . Fingerprinting Acute Digestive Diseases by Untargeted NMR Based Metabolomics. Int J Mol Sci. 2018; 19(11). PMC: 6274841. DOI: 10.3390/ijms19113288. View

Cen M, Wang F, Su Y, Zhang W, Sun B, Wang G . Gastrointestinal microecology: a crucial and potential target in acute pancreatitis. Apoptosis. 2018; 23(7-8):377-387. DOI: 10.1007/s10495-018-1464-9. View

Fiehn O . Metabolomics--the link between genotypes and phenotypes. Plant Mol Biol. 2002; 48(1-2):155-71. View

Wang D, Guo S, He H, Gong L, Cui H . Gut Microbiome and Serum Metabolome Analyses Identify Unsaturated Fatty Acids and Butanoate Metabolism Induced by Gut Microbiota in Patients With Chronic Spontaneous Urticaria. Front Cell Infect Microbiol. 2020; 10:24. PMC: 7047433. DOI: 10.3389/fcimb.2020.00024. View

LINDSAY D . Amino acids as energy sources. Proc Nutr Soc. 1980; 39(1):53-9. DOI: 10.1079/pns19800008. View

10.

Mei Q, Hu J, Huang Z, Fan J, Huang C, Lu Y . Pretreatment with chitosan oligosaccharides attenuate experimental severe acute pancreatitis via inhibiting oxidative stress and modulating intestinal homeostasis. Acta Pharmacol Sin. 2021; 42(6):942-953. PMC: 8149410. DOI: 10.1038/s41401-020-00581-5. View

11.

Li C, Wu S, Sun L, Yan T, Wang Y . Protective Effect of Zengye Decoction () on Submandibular Glands in Nonobese Diabetic Mice. Chin J Integr Med. 2014; 25(1):45-50. DOI: 10.1007/s11655-014-1981-5. View

12.

Uchino S, Kellum J, Bellomo R, Doig G, Morimatsu H, Morgera S . Acute renal failure in critically ill patients: a multinational, multicenter study. JAMA. 2005; 294(7):813-8. DOI: 10.1001/jama.294.7.813. View

13.

Bermingham E, MacLean P, Thomas D, Cave N, Young W . Key bacterial families (Clostridiaceae, Erysipelotrichaceae and Bacteroidaceae) are related to the digestion of protein and energy in dogs. PeerJ. 2017; 5:e3019. PMC: 5337088. DOI: 10.7717/peerj.3019. View

14.

Arutla M, Raghunath M, Deepika G, Jakkampudi A, Murthy H, Rao G . Efficacy of enteral glutamine supplementation in patients with severe and predicted severe acute pancreatitis- A randomized controlled trial. Indian J Gastroenterol. 2019; 38(4):338-347. DOI: 10.1007/s12664-019-00962-7. View

15.

Li J, Zhu S, Zhao X, Liu Y, Wan M, Guo H . Metabolomic profiles illuminate the efficacy of Chinese herbal Da-Cheng-Qi decoction on acute pancreatitis in rats. Pancreatology. 2015; 15(4):337-43. DOI: 10.1016/j.pan.2015.04.011. View

16.

Razmpoosh E, Safi S, Abdollahi N, Nadjarzadeh A, Nazari M, Fallahzadeh H . The effect of Nigella sativa on the measures of liver and kidney parameters: A systematic review and meta-analysis of randomized-controlled trials. Pharmacol Res. 2020; 156:104767. DOI: 10.1016/j.phrs.2020.104767. View

17.

Banks P, Bollen T, Dervenis C, Gooszen H, Johnson C, Sarr M . Classification of acute pancreatitis--2012: revision of the Atlanta classification and definitions by international consensus. Gut. 2012; 62(1):102-11. DOI: 10.1136/gutjnl-2012-302779. View

18.

Wu T, Xu F, Su C, Li H, Lv N, Liu Y . Alterations in the Gut Microbiome and Cecal Metabolome During -Induced Pneumosepsis. Front Immunol. 2020; 11:1331. PMC: 7411141. DOI: 10.3389/fimmu.2020.01331. View

19.

Rooks M, Garrett W . Gut microbiota, metabolites and host immunity. Nat Rev Immunol. 2016; 16(6):341-52. PMC: 5541232. DOI: 10.1038/nri.2016.42. View

20.

Chang S, Sun R, Feng M, Li Y, Wang H, Xu Y . BML-111 inhibits the inflammatory response and apoptosis of renal tissue in rats with hemorrhagic shock by inhibiting the MAPK pathway. Eur Rev Med Pharmacol Sci. 2018; 22(11):3439-3447. DOI: 10.26355/eurrev_201806_15168. View