Induced Mutagenesis in Dam- Mutants of Escherichia Coli: a Role for 6-methyladenine Residues in Mutation Avoidance

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Jul 25

PMID 355857

Citations 81

Authors

B Glickman

P van den Elsen

M Radman

Affiliations

Soon will be listed here.

Abstract

E. coli strains carrying the dam-3 and dam-4 mutations resulting in reduced levels of 6-methyladenine in the DNA have been found to be more sensitive to base analogue mutagenesis than dam+ strains. Mutagenesis by EMS was also found to be enhanced in dam- strains. Dam- mutants however were not found to be hypermutable by UV light. It is concluded that the dam- strains are deficient in the correct repair of mispairing lesions. The data are consistent with the hypothesis that 6-methyladenine residues in the DNA are involved in strand discrimination during mismatch correction.

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