in Dogs and Humans in Slovenia

Overview

Journal J Vet Res

Publisher Sciendo

Specialty Veterinary Medicine

Date 2022 May 18

PMID 35582489

Authors

Tina Kotnik

Aleksandra Vergles Rataj

Barbara Soba

Affiliations

Soon will be listed here.

Abstract

Introduction: The prevalence of in dogs in countries bordering Slovenia ranges from 1.5% to 47.3%. The aim of this study was to estimate its prevalence in Slovenian dogs and to present the cases of dirofilariasis diagnosed in humans from 2010 to 2020.

Material And Methods: Epidemiological data were collected and blood samples were taken from 465 dogs older than one year and born in Slovenia. A real-time PCR was performed on all samples to detect filarioid DNA, and a -and -specific real-time PCR was performed on positive samples. Blood samples from 446 dogs were tested for spp. using a modified Knott's test. Human cases were diagnosed from histological sections of excised subcutaneous nodules. Descriptive statistics were used to characterise the samples. The one-sample nonparametric chi-squared test was used to assess whether categories of a variable were equally distributed.

Results: Three dogs' samples tested positive for using the species-specific real-time PCR, while DNA was not detected. The modified Knott's test was positive in two of the three PCR-positive dogs, two of which had never travelled outside Slovenia's borders. Four human patients with dirofilariasis were diagnosed. Since their travel history was unknown, autochthonous transmission could not be confirmed.

Conclusion: Our study demonstrated a 0.64% prevalence of infection in dogs in Slovenia. Two cases could be autochthonous.

Citing Articles

Severe and Rare Case of Human Dirofilaria repens Infection with Pleural and Subcutaneous Manifestations, Slovenia.

Biasizzo H, Soba B, Ilovski F, Harlander M, Lukin M, Blatnik O Emerg Infect Dis. 2022; 28(12):2504-2507.

PMID: 36417956 PMC: 9707586. DOI: 10.3201/eid2812.221366.

References

Pampiglione S, Rivasi F . Human dirofilariasis due to Dirofilaria (Nochtiella) repens: an update of world literature from 1995 to 2000. Parassitologia. 2001; 42(3-4):231-54. View

Tarello W . Clinical Aspects of Dermatitis Associated with Dirofilaria repens in Pets: A Review of 100 Canine and 31 Feline Cases (1990-2010) and a Report of a New Clinic Case Imported from Italy to Dubai. J Parasitol Res. 2011; 2011:578385. PMC: 3238394. DOI: 10.1155/2011/578385. View

Pantchev N, Etzold M, Daugschies A, Dyachenko V . Diagnosis of imported canine filarial infections in Germany 2008 - 2010. Parasitol Res. 2011; 109 Suppl 1:S61-76. DOI: 10.1007/s00436-011-2403-7. View

Sonnberger K, Duscher G, Fuehrer H, Leschnik M . Current trends in canine dirofilariosis in Austria-do we face a pre-endemic status?. Parasitol Res. 2020; 119(3):1001-1009. PMC: 7075843. DOI: 10.1007/s00436-019-06576-4. View

Tarello W . Cutaneous lesions in dogs with Dirofilaria (Nochtiella) repens infestation and concurrent tick-borne transmitted diseases. Vet Dermatol. 2002; 13(5):267-74. DOI: 10.1046/j.1365-3164.2002.00305.x. View

Bajer A, Mierzejewska E, Rodo A, Bednarska M, Kowalec M, Welc-Faleciak R . The risk of vector-borne infections in sled dogs associated with existing and new endemic areas in Poland: Part 1: A population study on sled dogs during the racing season. Vet Parasitol. 2014; 202(3-4):276-86. DOI: 10.1016/j.vetpar.2013.12.033. View

Capelli G, Genchi C, Baneth G, Bourdeau P, Brianti E, Cardoso L . Recent advances on Dirofilaria repens in dogs and humans in Europe. Parasit Vectors. 2018; 11(1):663. PMC: 6299983. DOI: 10.1186/s13071-018-3205-x. View

Latrofa M, Montarsi F, Ciocchetta S, Annoscia G, Dantas-Torres F, Ravagnan S . Molecular xenomonitoring of Dirofilaria immitis and Dirofilaria repens in mosquitoes from north-eastern Italy by real-time PCR coupled with melting curve analysis. Parasit Vectors. 2012; 5:76. PMC: 3438040. DOI: 10.1186/1756-3305-5-76. View

Gabrielli S, Mangano V, Furzi F, Oliva A, Vita S, Poscia R . Molecular Identification of New Cases of Human Dirofilariosis () in Italy. Pathogens. 2021; 10(2). PMC: 7926647. DOI: 10.3390/pathogens10020251. View

10.

Laidoudi Y, Davoust B, Varloud M, Niang E, Fenollar F, Mediannikov O . Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum. Parasit Vectors. 2020; 13(1):319. PMC: 7309989. DOI: 10.1186/s13071-020-04185-0. View

11.

Sabunas V, Radzijevskaja J, Sakalauskas P, Petkevicius S, Karveliene B, Ziliukiene J . Dirofilaria repens in dogs and humans in Lithuania. Parasit Vectors. 2019; 12(1):177. PMC: 6472076. DOI: 10.1186/s13071-019-3406-y. View

12.

Laidoudi Y, Marie J, Tahir D, Watier-Grillot S, Mediannikov O, Davoust B . Detection of Canine Vector-Borne Filariasis and Their Endosymbionts in French Guiana. Microorganisms. 2020; 8(5). PMC: 7285362. DOI: 10.3390/microorganisms8050770. View

13.

Farkas R, Mag V, Gyurkovszky M, Takacs N, Voros K, Solymosi N . The current situation of canine dirofilariosis in Hungary. Parasitol Res. 2019; 119(1):129-135. PMC: 6942023. DOI: 10.1007/s00436-019-06478-5. View

14.

Rojas A, Rojas D, Montenegro V, Baneth G . Detection of Dirofilaria immitis and other arthropod-borne filarioids by an HRM real-time qPCR, blood-concentrating techniques and a serological assay in dogs from Costa Rica. Parasit Vectors. 2015; 8:170. PMC: 4377020. DOI: 10.1186/s13071-015-0783-8. View

15.

Kotnik T, Moreno J, Soba B, Krt B, Skvarc M, Rataj A . Canine Leishmaniasis Prevalence in the Slovenian Dog Population. J Vet Res. 2021; 65(2):161-167. PMC: 8256472. DOI: 10.2478/jvetres-2021-0028. View

16.

Tahir D, Davoust B, Parola P . Vector-borne nematode diseases in pets and humans in the Mediterranean Basin: An update. Vet World. 2019; 12(10):1630-1643. PMC: 6868252. DOI: 10.14202/vetworld.2019.1630-1643. View

17.

Tarello W . Dermatitis associated with Dirofilaria repens microfilariae in a dog from Rome. Vet J. 2003; 165(2):175-7. DOI: 10.1016/s1090-0233(02)00243-5. View

18.

Logar J, Novsak V, Rakovec S, Stanisa O . Subcutaneous infection caused by Dirofilaria repens imported to Slovenia. J Infect. 2001; 42(1):72-4. DOI: 10.1053/jinf.2000.0759. View

19.

Tasic-Otasevic S, Trenkic Bozinovic M, Gabrielli S, Genchi C . Canine and human Dirofilaria infections in the Balkan Peninsula. Vet Parasitol. 2015; 209(3-4):151-6. DOI: 10.1016/j.vetpar.2015.02.016. View

20.

Pupic-Bakrac A, Pupic-Bakrac J, Jurkovic D, Capar M, Lazaric Stefanovic L, Antunovic Celovic I . The trends of human dirofilariasis in Croatia: Yesterday - Today - Tomorrow. One Health. 2020; 10:100153. PMC: 7582219. DOI: 10.1016/j.onehlt.2020.100153. View