Two-Photon-Excited Single-Molecule Fluorescence Enhanced by Gold Nanorod Dimers

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2022 May 16

PMID 35575461

Authors

Xuxing Lu

Deep Punj

Michel Orrit

Affiliations

Soon will be listed here.

Abstract

We demonstrate two-photon-excited single-molecule fluorescence enhancement by single end-to-end self-assembled gold nanorod dimers. We employed biotinylated streptavidin as the molecular linker, which connected two gold nanorods in end-to-end fashion. The typical size of streptavidin of around 5 nm separates the gold nanorods with gaps suitable for the access of fresh dyes in aqueous solution, yet small enough to give very high two-photon fluorescence enhancement. Simulations show that enhancements of more than 7 orders of magnitude can be achieved for two-photon-excited fluorescence in the plasmonic hot spots. With such high enhancements, we successfully detect two-photon-excited fluorescence for a common organic dye (ATTO 610) at the single-molecule, single-nanoparticle level.

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