Betaine Regulates the Production of Reactive Oxygen Species Through Wnt10b Signaling in the Liver of Zebrafish

Overview

Journal Front Physiol

Date 2022 May 16

PMID 35574489

Authors

Ao Li

Yaqi Gu

Xiuzhen Zhang

Hairui Yu

Dongwu Liu

Qiuxiang Pang

Affiliations

Soon will be listed here.

Abstract

When fish are under oxidative stress, levels of reactive oxygen species (ROS) are chronically elevated, which play a crucial role in fish innate immunity. In the present study, the mechanism by which betaine regulates ROS production Wnt10b/β-catenin signaling was investigated in zebrafish liver. Our results showed that betaine enrichment of diet at 0.1, 0.2 and 0.4 g/kg induced Wnt10b and β-catenin gene expression, but suppressed GSK-3β expression in zebrafish liver. In addition, the content of superoxide anion (O ), hydrogen peroxide (HO) and hydroxyl radical (·OH) was decreased by all of the experimental betaine treatments. However, betaine enrichment of diet at 0.1, 0.2 and 0.4 g/kg enhanced gene expression and activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) in zebrafish liver. In addition, Wnt10b RNA was further interfered in zebrafish, and the results of Wnt10b RNAi indicated that Wnt10b plays a key role in regulating ROS production and antioxidant enzyme activity. In conclusion, betaine can inhibit ROS production in zebrafish liver through the Wnt10b/β-catenin signaling pathway.

Citing Articles

Effect of Polystyrene Microplastics on the Antioxidant System and Immune Response in GIFT ().

Zheng Y, Addotey T, Chen J, Xu G Biology (Basel). 2023; 12(11).

PMID: 37998029 PMC: 10669825. DOI: 10.3390/biology12111430.

New alternative ingredients and genetic selection are the next game changers in rainbow trout nutrition: a metabolomics appraisal.

Roques S, Deborde C, Skiba-Cassy S, Medale F, Dupont-Nivet M, LeFevre F Sci Rep. 2023; 13(1):19634.

PMID: 37949954 PMC: 10638236. DOI: 10.1038/s41598-023-46809-2.

Molecular Mechanism of m6A Methylation Modification Genes and in Regulating Heat Stress in Sheep.

Chen B, Yuan C, Guo T, Liu J, Yang B, Lu Z Int J Mol Sci. 2023; 24(15).

PMID: 37569302 PMC: 10419070. DOI: 10.3390/ijms241511926.

References

Kuang S, Xiao W, Feng L, Liu Y, Jiang J, Jiang W . Effects of graded levels of dietary methionine hydroxy analogue on immune response and antioxidant status of immune organs in juvenile Jian carp (Cyprinus carpio var. Jian). Fish Shellfish Immunol. 2012; 32(5):629-36. DOI: 10.1016/j.fsi.2011.12.012. View

Molenaar M, van de Wetering M, Oosterwegel M, Peterson-Maduro J, Godsave S, Korinek V . XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos. Cell. 1996; 86(3):391-9. DOI: 10.1016/s0092-8674(00)80112-9. View

Ridgeway A, Petropoulos H, Wilton S, Skerjanc I . Wnt signaling regulates the function of MyoD and myogenin. J Biol Chem. 2000; 275(42):32398-405. DOI: 10.1074/jbc.M004349200. View

Cossu G, Borello U . Wnt signaling and the activation of myogenesis in mammals. EMBO J. 1999; 18(24):6867-72. PMC: 1171749. DOI: 10.1093/emboj/18.24.6867. View

Craig S . Betaine in human nutrition. Am J Clin Nutr. 2004; 80(3):539-49. DOI: 10.1093/ajcn/80.3.539. View

Taelman V, Dobrowolski R, Plouhinec J, Fuentealba L, Vorwald P, Gumper I . Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. Cell. 2010; 143(7):1136-48. PMC: 3022472. DOI: 10.1016/j.cell.2010.11.034. View

Giris M, Dogru-Abbasoglu S, Soluk-Tekkesin M, Olgac V, Uysal M . Effect of betaine treatment on the regression of existing hepatic triglyceride accumulation and oxidative stress in rats fed on high fructose diet. Gen Physiol Biophys. 2018; 37(5):563-570. DOI: 10.4149/gpb_2018005. View

Eklund M, Bauer E, Wamatu J, Mosenthin R . Potential nutritional and physiological functions of betaine in livestock. Nutr Res Rev. 2008; 18(1):31-48. DOI: 10.1079/NRR200493. View

Bhanot P, Brink M, Samos C, Hsieh J, Wang Y, Macke J . A new member of the frizzled family from Drosophila functions as a Wingless receptor. Nature. 1996; 382(6588):225-30. DOI: 10.1038/382225a0. View

10.

Bagnasco S, Balaban R, Fales H, Yang Y, Burg M . Predominant osmotically active organic solutes in rat and rabbit renal medullas. J Biol Chem. 1986; 261(13):5872-7. View

11.

Logan C, Nusse R . The Wnt signaling pathway in development and disease. Annu Rev Cell Dev Biol. 2004; 20:781-810. DOI: 10.1146/annurev.cellbio.20.010403.113126. View

12.

Petronini P, De Angelis E, Borghetti P, Borghetti A, WHEELER K . Modulation by betaine of cellular responses to osmotic stress. Biochem J. 1992; 282 ( Pt 1):69-73. PMC: 1130890. DOI: 10.1042/bj2820069. View

13.

Naik E, Dixit V . Mitochondrial reactive oxygen species drive proinflammatory cytokine production. J Exp Med. 2011; 208(3):417-20. PMC: 3058577. DOI: 10.1084/jem.20110367. View

14.

Hall L, Dunshea F, Allen J, Rungruang S, Collier J, Long N . Evaluation of dietary betaine in lactating Holstein cows subjected to heat stress. J Dairy Sci. 2016; 99(12):9745-9753. DOI: 10.3168/jds.2015-10514. View

15.

Saeed M, Babazadeh D, Naveed M, Arain M, Hassan F, Chao S . Reconsidering betaine as a natural anti-heat stress agent in poultry industry: a review. Trop Anim Health Prod. 2017; 49(7):1329-1338. DOI: 10.1007/s11250-017-1355-z. View

16.

Zhou Y, Holmseth S, Hua R, Lehre A, Olofsson A, Poblete-Naredo I . The betaine-GABA transporter (BGT1, slc6a12) is predominantly expressed in the liver and at lower levels in the kidneys and at the brain surface. Am J Physiol Renal Physiol. 2011; 302(3):F316-28. DOI: 10.1152/ajprenal.00464.2011. View

17.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

18.

Zhang L, Ying S, An W, Lian H, Zhou G, Han Z . Effects of dietary betaine supplementation subjected to heat stress on milk performances and physiology indices in dairy cow. Genet Mol Res. 2014; 13(3):7577-86. DOI: 10.4238/2014.September.12.25. View

19.

Wen C, Chen Y, Leng Z, Ding L, Wang T, Zhou Y . Dietary betaine improves meat quality and oxidative status of broilers under heat stress. J Sci Food Agric. 2018; 99(2):620-623. DOI: 10.1002/jsfa.9223. View

20.

Zhang M, Wu X, Lai F, Zhang X, Wu H, Min T . Betaine Inhibits Hepatitis B Virus with an Advantage of Decreasing Resistance to Lamivudine and Interferon α. J Agric Food Chem. 2016; 64(20):4068-77. DOI: 10.1021/acs.jafc.6b01180. View