MEF2C Expression Is Regulated by the Post-transcriptional Activation of the METTL3-mA-YTHDF1 Axis in Myoblast Differentiation

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2022 May 16

PMID 35573410

Authors

Xinran Yang

Yue Ning

Sayed Haidar Abbas Raza

Chugang Mei

Linsen Zan

Affiliations

Soon will be listed here.

Abstract

-methyladenosine (mA) plays an essential role in regulating gene expression. However, the effect of mA on skeletal myoblast differentiation and the underlying mechanisms are still unclear. Here, we ascertained mRNA mA methylation exhibited declined changes during bovine skeletal myoblast differentiation, and both mRNA expression and mA levels were significantly increased during myoblast differentiation. We found that with mutated mA sites significantly inhibited myoblast differentiation compared with wild-type . METTL3 promoted MEF2C protein expression through posttranscriptional modification in an mA-YTHDF1-dependent manner. Moreover, MEF2C promoted the expression of METTL3 by binding to its promoter. These results revealed that there is a positive feedback loop between these molecules in myoblast differentiation. Our study provided new insights into skeletal muscle differentiation and fusion, which may provide an RNA methylation-based approach for molecular genetics and breeding in livestock as well as for the treatment of muscle-related diseases.

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