Liver Receptor Homolog-1 Regulates Apoptosis of Bovine Ovarian Granulosa Cells by Progestogen Receptor Signaling Pathway

Overview

Journal Animals (Basel)

Date 2022 May 14

PMID 35565639

Authors

Dejun Xu

Xiaohan Jiang

Yukun Wang

Shuaifei Song

Affiliations

Soon will be listed here.

Abstract

The purpose of the present investigation was to assess the function of LRH-1 on GCs and the mechanisms involved. Here, LRH- was highly expressed in the bovine GCs of atretic follicles. Treatment with 50 μM of LRH-1 agonist (DLPC) significantly induced the expression of LRH-1 (p < 0.05). In particular, LRH-1 activation blocked the progestogen receptor signaling pathway via downregulating progesterone production and progestogen receptor levels (p < 0.05), but had no effect on 17 beta-estradiol synthesis. Meanwhile, LRH-1 activation promoted the apoptosis of GCs and increased the activity of caspase 3 (p < 0.05). Importantly, upregulating the progestogen receptor signaling pathway with progestogen could attenuate the LRH-1-induced proapoptotic effect. Moreover, treatment with progestogen decreased the activity of the proapoptotic gene caspase 3 and increased the expression of antiapoptotic gene Bcl2 in LRH-1 activated GCs (p < 0.05). Taken together, these results demonstrate that LRH-1 might be dependent on the progestogen receptor signaling pathway to modulate bovine follicular atresia.

Citing Articles

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Wu T, Lu Z, Yu H, Wu X, Liu Y, Xu Y Acta Pharmacol Sin. 2024; 45(8):1571-1581.

PMID: 38632319 PMC: 11272790. DOI: 10.1038/s41401-024-01276-x.

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