Polymerizable Skin Hydrogel for Full Thickness Wound Healing

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 14

PMID 35563225

Authors

Mairobi Persinal-Medina

Sara Llames

Manuel Chacon

Natalia Vazquez

Marta Pevida

Ignacio Alcalde

Sergio Alonso-Alonso

Laura Maria Martinez-Lopez

Jesus Merayo-Lloves

Alvaro Meana

Affiliations

Soon will be listed here.

Abstract

The skin is the largest organ in the human body, comprising the main barrier against the environment. When the skin loses its integrity, it is critical to replace it to prevent water loss and the proliferation of opportunistic infections. For more than 40 years, tissue-engineered skin grafts have been based on the in vitro culture of keratinocytes over different scaffolds, requiring between 3 to 4 weeks of tissue culture before being used clinically. In this study, we describe the development of a polymerizable skin hydrogel consisting of keratinocytes and fibroblast entrapped within a fibrin scaffold. We histologically characterized the construct and evaluated its use on an in vivo wound healing model of skin damage. Our results indicate that the proposed methodology can be used to effectively regenerate skin wounds, avoiding the secondary in vitro culture steps and thus, shortening the time needed until transplantation in comparison with other bilayer skin models. This is achievable due to the instant polymerization of the keratinocytes and fibroblast combination that allows a direct application on the wound. We suggest that the polymerizable skin hydrogel is an inexpensive, easy and rapid treatment that could be transferred into clinical practice in order to improve the treatment of skin wounds.

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References

Ettinger A, Miklauz M, Hendrix B, Bihm D, Maldonado-Codina G, Goodrich R . Protein stability of previously frozen plasma, riboflavin and UV light-treated, refrozen and stored for up to 2 years at -30 °C. Transfus Apher Sci. 2011; 44(1):25-31. DOI: 10.1016/j.transci.2010.12.005. View

Atiyeh B, Costagliola M . Cultured epithelial autograft (CEA) in burn treatment: three decades later. Burns. 2007; 33(4):405-13. DOI: 10.1016/j.burns.2006.11.002. View

Ganguly S, Das P, Itzhaki E, Hadad E, Gedanken A, Margel S . Microwave-Synthesized Polysaccharide-Derived Carbon Dots as Therapeutic Cargoes and Toughening Agents for Elastomeric Gels. ACS Appl Mater Interfaces. 2020; 12(46):51940-51951. DOI: 10.1021/acsami.0c14527. View

Badylak S . Xenogeneic extracellular matrix as a scaffold for tissue reconstruction. Transpl Immunol. 2004; 12(3-4):367-77. DOI: 10.1016/j.trim.2003.12.016. View

Whitman D, Berry R, Green D . Platelet gel: an autologous alternative to fibrin glue with applications in oral and maxillofacial surgery. J Oral Maxillofac Surg. 1997; 55(11):1294-9. DOI: 10.1016/s0278-2391(97)90187-7. View

Ruszymah B . Autologous human fibrin as the biomaterial for tissue engineering. Med J Malaysia. 2004; 59 Suppl B:30-1. View

Ma L, Gao C, Mao Z, Zhou J, Shen J, Hu X . Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials. 2003; 24(26):4833-41. DOI: 10.1016/s0142-9612(03)00374-0. View

Agarwal A, Kumar D, Jacob S, Baid C, Agarwal A, Srinivasan S . Fibrin glue-assisted sutureless posterior chamber intraocular lens implantation in eyes with deficient posterior capsules. J Cataract Refract Surg. 2008; 34(9):1433-8. DOI: 10.1016/j.jcrs.2008.04.040. View

Mazlyzam A, Aminuddin B, Fuzina N, Norhayati M, Fauziah O, Isa M . Reconstruction of living bilayer human skin equivalent utilizing human fibrin as a scaffold. Burns. 2007; 33(3):355-63. DOI: 10.1016/j.burns.2006.08.022. View

10.

Meana A, Iglesias J, Madrigal B, Sanchez J . Use of cyanoacrylate glue to prepare cultured keratinocyte sheets for grafting. Burns. 1998; 23(7-8):645-6. DOI: 10.1016/s0305-4179(97)00077-6. View

11.

Sierra D . Fibrin sealant adhesive systems: a review of their chemistry, material properties and clinical applications. J Biomater Appl. 1993; 7(4):309-52. DOI: 10.1177/088532829300700402. View

12.

Rheinwald J, Green H . Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell. 1975; 6(3):331-43. DOI: 10.1016/s0092-8674(75)80001-8. View

13.

Yang B, Song J, Jiang Y, Li M, Wei J, Qin J . Injectable Adhesive Self-Healing Multicross-Linked Double-Network Hydrogel Facilitates Full-Thickness Skin Wound Healing. ACS Appl Mater Interfaces. 2020; 12(52):57782-57797. DOI: 10.1021/acsami.0c18948. View

14.

Liang Y, Zhao X, Hu T, Chen B, Yin Z, Ma P . Adhesive Hemostatic Conducting Injectable Composite Hydrogels with Sustained Drug Release and Photothermal Antibacterial Activity to Promote Full-Thickness Skin Regeneration During Wound Healing. Small. 2019; 15(12):e1900046. DOI: 10.1002/smll.201900046. View

15.

Llames S, Garcia E, Garcia V, Del Rio M, Larcher F, Jorcano J . Clinical results of an autologous engineered skin. Cell Tissue Bank. 2006; 7(1):47-53. DOI: 10.1007/s10561-004-7253-4. View

16.

Gomez C, Galan J, Torrero V, Ferreiro I, Perez D, Palao R . Use of an autologous bioengineered composite skin in extensive burns: Clinical and functional outcomes. A multicentric study. Burns. 2011; 37(4):580-9. DOI: 10.1016/j.burns.2010.10.005. View

17.

Wood F, Kolybaba M, Allen P . The use of cultured epithelial autograft in the treatment of major burn injuries: a critical review of the literature. Burns. 2006; 32(4):395-401. DOI: 10.1016/j.burns.2006.01.008. View

18.

Clauss A . [Rapid physiological coagulation method in determination of fibrinogen]. Acta Haematol. 1957; 17(4):237-46. DOI: 10.1159/000205234. View

19.

Vig K, Chaudhari A, Tripathi S, Dixit S, Sahu R, Pillai S . Advances in Skin Regeneration Using Tissue Engineering. Int J Mol Sci. 2017; 18(4). PMC: 5412373. DOI: 10.3390/ijms18040789. View

20.

Ahmed T, Dare E, Hincke M . Fibrin: a versatile scaffold for tissue engineering applications. Tissue Eng Part B Rev. 2008; 14(2):199-215. DOI: 10.1089/ten.teb.2007.0435. View