Insights into the Molecular Regulation of Lignin Content in Triploid Poplar Leaves

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 14

PMID 35562994

Authors

Tingting Xu

Shuwen Zhang

Kang Du

Jun Yang

Xiangyang Kang

Affiliations

Soon will be listed here.

Abstract

After polyploidization, plants usually undergo some morphological and physiological changes, including the lignin content of polyploids usually becoming lower than that of diploids. However, the regulatory mechanism of the variation of lignin content in polyploid plants remains unclear. Therefore, in this research, we used full-sib poplar triploids and diploids to explore the molecular regulatory basis of lignin content in poplar triploid leaves through the determination of lignin content, the observation of xylem cells, and transcriptome sequencing. The results showed that the lignin content of triploid leaves was significantly lower than that of diploid leaves. The xylem cells of triploid leaves were significantly larger than those of diploids. Transcriptome sequencing data show that most lignin biosynthesis genes were significantly downregulated, and genes related to cell growth were mostly upregulated in triploid leaves compared with diploid leaves. In addition, co-expression network analysis showed that several transcription factors might be involved in the regulation of lignin biosynthesis. Consequently, the altered expression of genes related to lignin might lead to the reduced lignin content in triploids. These results provide a theoretical basis for further exploring the molecular mechanism of the variation of polyploid lignin content and the utilization of polyploid lignocellulosic resources.

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References

Zhang Q, Xie Z, Zhang R, Xu P, Liu H, Yang H . Blue Light Regulates Secondary Cell Wall Thickening via MYC2/MYC4 Activation of the -Directed Transcriptional Network in Arabidopsis. Plant Cell. 2018; 30(10):2512-2528. PMC: 6241271. DOI: 10.1105/tpc.18.00315. View

Foster C, Martin T, Pauly M . Comprehensive compositional analysis of plant cell walls (Lignocellulosic biomass) part I: lignin. J Vis Exp. 2010; (37). PMC: 3144576. DOI: 10.3791/1745. View

Wang L, Ran L, Hou Y, Tian Q, Li C, Liu R . The transcription factor MYB115 contributes to the regulation of proanthocyanidin biosynthesis and enhances fungal resistance in poplar. New Phytol. 2017; 215(1):351-367. DOI: 10.1111/nph.14569. View

Fornale S, Capellades M, Encina A, Wang K, Irar S, Lapierre C . Altered lignin biosynthesis improves cellulosic bioethanol production in transgenic maize plants down-regulated for cinnamyl alcohol dehydrogenase. Mol Plant. 2011; 5(4):817-30. DOI: 10.1093/mp/ssr097. View

Anders S, Huber W . Differential expression analysis for sequence count data. Genome Biol. 2010; 11(10):R106. PMC: 3218662. DOI: 10.1186/gb-2010-11-10-r106. View

Li Q, Song J, Peng S, Wang J, Qu G, Sederoff R . Plant biotechnology for lignocellulosic biofuel production. Plant Biotechnol J. 2014; 12(9):1174-92. DOI: 10.1111/pbi.12273. View

Wang J, Chuang L, Loziuk P, Chen H, Lin Y, Shi R . Phosphorylation is an on/off switch for 5-hydroxyconiferaldehyde O-methyltransferase activity in poplar monolignol biosynthesis. Proc Natl Acad Sci U S A. 2015; 112(27):8481-6. PMC: 4500226. DOI: 10.1073/pnas.1510473112. View

Allario T, Brumos J, Colmenero-Flores J, Tadeo F, Froelicher Y, Talon M . Large changes in anatomy and physiology between diploid Rangpur lime (Citrus limonia) and its autotetraploid are not associated with large changes in leaf gene expression. J Exp Bot. 2011; 62(8):2507-19. DOI: 10.1093/jxb/erq467. View

Warner D, Edwards G . Effects of polyploidy on photosynthesis. Photosynth Res. 2013; 35(2):135-47. DOI: 10.1007/BF00014744. View

10.

Liu Q, Luo L, Zheng L . Lignins: Biosynthesis and Biological Functions in Plants. Int J Mol Sci. 2018; 19(2). PMC: 5855557. DOI: 10.3390/ijms19020335. View

11.

Draghici S, Khatri P, Tarca A, Amin K, Done A, Voichita C . A systems biology approach for pathway level analysis. Genome Res. 2007; 17(10):1537-45. PMC: 1987343. DOI: 10.1101/gr.6202607. View

12.

Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, Richard T . Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol. 2005; 140(2):499-511. PMC: 1361319. DOI: 10.1104/pp.105.067231. View

13.

Zhong R, Ye Z . Complexity of the transcriptional network controlling secondary wall biosynthesis. Plant Sci. 2014; 229:193-207. DOI: 10.1016/j.plantsci.2014.09.009. View

14.

Wang X, Wu J, Guan M, Zhao C, Geng P, Zhao Q . Arabidopsis MYB4 plays dual roles in flavonoid biosynthesis. Plant J. 2019; 101(3):637-652. DOI: 10.1111/tpj.14570. View

15.

Langmead B, Trapnell C, Pop M, Salzberg S . Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009; 10(3):R25. PMC: 2690996. DOI: 10.1186/gb-2009-10-3-r25. View

16.

Yoon J, Choi H, An G . Roles of lignin biosynthesis and regulatory genes in plant development. J Integr Plant Biol. 2015; 57(11):902-12. PMC: 5111759. DOI: 10.1111/jipb.12422. View

17.

Wagner A, Tobimatsu Y, Phillips L, Flint H, Torr K, Donaldson L . CCoAOMT suppression modifies lignin composition in Pinus radiata. Plant J. 2011; 67(1):119-29. DOI: 10.1111/j.1365-313X.2011.04580.x. View

18.

Voelker S, Lachenbruch B, Meinzer F, Strauss S . Reduced wood stiffness and strength, and altered stem form, in young antisense 4CL transgenic poplars with reduced lignin contents. New Phytol. 2010; 189(4):1096-1109. DOI: 10.1111/j.1469-8137.2010.03572.x. View

19.

Ashburner M, Ball C, Blake J, Botstein D, Butler H, Cherry J . Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet. 2000; 25(1):25-9. PMC: 3037419. DOI: 10.1038/75556. View

20.

Wagner A, Tobimatsu Y, Phillips L, Flint H, Geddes B, Lu F . Syringyl lignin production in conifers: Proof of concept in a Pine tracheary element system. Proc Natl Acad Sci U S A. 2015; 112(19):6218-23. PMC: 4434704. DOI: 10.1073/pnas.1411926112. View