LncRNA Promotes Muscle Hypertrophy and Fast-Twitch Fiber Transformation Via Enhancing Expression

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 14

PMID 35562902

Authors

Jing Zhang

Bolin Cai

Manting Ma

Shaofen Kong

Zhen Zhou

Xiquan Zhang

Qinghua Nie

Affiliations

Soon will be listed here.

Abstract

Long noncoding RNA (lncRNA) plays a crucial part in all kinds of life activities, especially in myogenesis. (SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin, subfamily d, member 3) is a member of the SWI/SNF protein complex and was reported to be required for cell proliferation and myoblast differentiation. In this study, we identified a new lncRNA named (), which strongly regulated the development of myogenesis by improving the expression of (). We overexpressed and knockdown the expression of and to investigate their function on myoblast proliferation and differentiation. Cell experiments proved that and promoted myoblast proliferation through the pathway and improved differentiation of differentiated myoblasts through the pathway. Moreover, they upregulated the fast-twitch fiber-related genes and downregulated the slow-twitch fiber-related genes, which indicated that they facilitated the slow-twitch fiber to transform into the fast-twitch fiber. The animals' experiments supported the results above, demonstrating that could induce muscle hypertrophy and fast-twitch fiber transformation. In conclusion, can improve the expression of , thus inducing muscle hypertrophy. In addition, can facilitate slow-twitch fibers to transform into fast-twitch fibers.

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