Discovery of Five New Ethylene-Forming Enzymes for Clean Production of Ethylene in

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 May 14

PMID 35562889

Authors

Yixuan Cui

Ying Jiang

Meng Xiao

Muhammad Zeeshan Munir

Sadaf Riaz

Faiz Rasul

Maurycy Daroch

Affiliations

Soon will be listed here.

Abstract

Ethylene is an essential platform chemical with a conjugated double bond, which can produce many secondary chemical products through copolymerisation. At present, ethylene production is mainly from petroleum fractionation and cracking, which are unsustainable in the long term, and harmful to our environment. Therefore, a hot research field is seeking a cleaner method for ethylene production. Based on the model ethylene-forming enzyme (Efe) AAD16440.1 (6vp4.1.A) from , we evaluated five putative Efe protein sequences using the data derived from phylogenetic analyses and the conservation of their catalytic structures. Then, pBAD expression frameworks were constructed, and relevant enzymes were expressed in BL21. Finally, enzymatic activity in vitro and in vivo was detected to demonstrate their catalytic activity. Our results show that the activity in vitro measured by the conversion of α-ketoglutarate was from 0.21-0.72 μmol ethylene/mg/min, which varied across the temperatures. In cells, the activity of the new Efes was 12.28-147.43 μmol/gDCW/h (DCW, dry cellular weight). Both results prove that all the five putative Efes could produce ethylene.

Citing Articles

Construction of an artificial consortium of and cyanobacteria for clean indirect production of volatile platform hydrocarbons from CO.

Cui Y, Rasul F, Jiang Y, Zhong Y, Zhang S, Boruta T Front Microbiol. 2022; 13:965968.

PMID: 36338098 PMC: 9635338. DOI: 10.3389/fmicb.2022.965968.

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