Fast Slow Folding of an Outer Membrane Porin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2022 May 13

PMID 35549548

Authors

Eve E Weatherill

Monifa A Fahie

David P Marshall

Rachel A Andvig

Matthew R Cheetham

Min Chen

Mark I Wallace

Affiliations

Soon will be listed here.

Abstract

In comparison to globular proteins, the spontaneous folding and insertion of β-barrel membrane proteins are surprisingly slow, typically occurring on the order of minutes. Using single-molecule Förster resonance energy transfer to report on the folding of fluorescently labeled outer membrane protein G we measured the real-time insertion of a β-barrel membrane protein from an unfolded state. Folding events were rare and fast (<20 ms), occurring immediately upon arrival at the membrane. This combination of infrequent, but rapid, folding resolves this apparent dichotomy between slow ensemble kinetics and the typical timescales of biomolecular folding.

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