LncRNA HAND2-AS1 Attenuates Glioma Cell Proliferation, Invasion and Migration by Targeting CDK6
Overview
Authors
Affiliations
Objective: Long non-coding RNA (lncRNA) HAND2-AS1 has been indicated to play biological roles in several cancers. However, whether HAND2-AS1 could exert its role in glioma remains unknown. We aimed to investigate the function of HAND2-AS1 in the proliferation, invasion and migration and to explore its underlying molecular mechanism in glioma cells.
Methods: Reverse transcription-quantitative polymerase chain reaction analysis was conducted to determine the expression of HAND2-AS1 and cyclin-dependent kinases 6 (CDK6) in tissues or cells. Western blot analysis was used to detect the CDK6 protein expression. CCK-8 assay was adopted to determine the proliferation in glioma cell lines. Transwell assay was taken to evaluate the invasion and migration in glioma cell lines.
Results: Our results revealed that HAND2-AS1 expression was significantly downregulated in the glioma tissues and cell lines. Moreover, overexpression of HAND2-AS1 attenuated the proliferation, invasion, and migration in glioma cell lines. However, overexpression of CDK6 could partially block the inhibitory role of HAND2-AS1 on cell proliferation as well as cell invasion and migration in glioma cell lines.
Conclusion: LncRNA HAND2-AS1 may play a critical anti-tumorigenic role in glioma by negatively regulating CDK6 expression.
HAND2-AS1 plays a tumor-suppressive role in hepatoblastoma through the negative regulation of CDK1.
Chen K, You Y, Tang W, Tian X, Zhu C, Yin Z Heliyon. 2024; 10(17):e35930.
PMID: 39286228 PMC: 11402935. DOI: 10.1016/j.heliyon.2024.e35930.
Vazana-Netzarim R, Elmalem Y, Sofer S, Bruck H, Danino N, Sarig U Int J Mol Sci. 2023; 24(22).
PMID: 38003736 PMC: 10672054. DOI: 10.3390/ijms242216546.
Cell Cycle-Related lncRNAs as Innovative Targets to Advance Cancer Management.
Liang X, Liu Y, Chen F, Zhou Z, Zhang L, Lin Z Cancer Manag Res. 2023; 15:547-561.
PMID: 37426392 PMC: 10327678. DOI: 10.2147/CMAR.S407371.
A review on the role of HAND2-AS1 in cancer.
Ghafouri-Fard S, Hussen B, Abdullah S, Dadyar M, Taheri M, Kiani A Clin Exp Med. 2023; 23(7):3179-3188.
PMID: 37204522 PMC: 10618356. DOI: 10.1007/s10238-023-01092-3.