Comparison of Different Pretreatments on the Synergistic Effect of Cellulase and Xylanase During the Enzymatic Hydrolysis of Sugarcane Bagasse

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 13

PMID 35548762

Authors

Chao Huang

Cheng Zhao

Hailong Li

Lian Xiong

Xuefang Chen

Mutan Luo

Xinde Chen

Affiliations

Soon will be listed here.

Abstract

Sugarcane bagasse (SCB) substrates with different chemical compositions were prepared by different pretreatments including dilute acid (DA), acidic sodium chlorite (ASC), alkali solution (AS), and alkali hydrogen peroxide (AHP). The compositions and chemical structures of pretreated SCB were characterized by HPLC, FTIR, XRD, and SEM. The addition of xylanase can significantly boost cellulase to hydrolyze cellulose and xylan especially for AS and AHP treated substrates. The obvious linear relationships between lignin removal and substrate digestibility were observed. ASC treated substrates obtained the highest digestibility (98.87%) of cellulose due to sufficiently removing lignin from SCB, whereas AHP treated substrates achieved the highest digestibility (84.61%) of xylan by cleaving the acetyl group on xylan and extending delignification. It was found that the synergistic effects between cellulase and xylanase were substrate and time specific. The better degree of synergy for the sugar production was in the initial hydrolysis stage but decreased in the later hydrolysis stage.

References

Mathew A, Parameshwaran B, Sukumaran R, Pandey A . An evaluation of dilute acid and ammonia fiber explosion pretreatment for cellulosic ethanol production. Bioresour Technol. 2015; 199:13-20. DOI: 10.1016/j.biortech.2015.08.121. View

Goncalves G, Takasugi Y, Jia L, Mori Y, Noda S, Tanaka T . Synergistic effect and application of xylanases as accessory enzymes to enhance the hydrolysis of pretreated bagasse. Enzyme Microb Technol. 2015; 72:16-24. DOI: 10.1016/j.enzmictec.2015.01.007. View

Qing Q, Zhou L, Huang M, Guo Q, He Y, Wang L . Improving enzymatic saccharification of bamboo shoot shell by alkalic salt pretreatment with H2O2. Bioresour Technol. 2015; 201:230-6. DOI: 10.1016/j.biortech.2015.11.059. View

Wang Y, De S, Yan N . Rational control of nano-scale metal-catalysts for biomass conversion. Chem Commun (Camb). 2016; 52(37):6210-24. DOI: 10.1039/c6cc00336b. View

Malgas S, Thoresen M, Susan van Dyk J, Pletschke B . Time dependence of enzyme synergism during the degradation of model and natural lignocellulosic substrates. Enzyme Microb Technol. 2017; 103:1-11. DOI: 10.1016/j.enzmictec.2017.04.007. View

Kumar L, Arantes V, Chandra R, Saddler J . The lignin present in steam pretreated softwood binds enzymes and limits cellulose accessibility. Bioresour Technol. 2011; 103(1):201-8. DOI: 10.1016/j.biortech.2011.09.091. View

Song H, Gao Y, Yang Y, Xiao W, Liu S, Xia W . Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates. Bioresour Technol. 2016; 219:710-715. DOI: 10.1016/j.biortech.2016.08.035. View

Zhang J, Viikari L . Impact of xylan on synergistic effects of xylanases and cellulases in enzymatic hydrolysis of lignocelluloses. Appl Biochem Biotechnol. 2014; 174(4):1393-1402. DOI: 10.1007/s12010-014-1140-7. View

Hu J, Arantes V, Pribowo A, Saddler J . The synergistic action of accessory enzymes enhances the hydrolytic potential of a "cellulase mixture" but is highly substrate specific. Biotechnol Biofuels. 2013; 6(1):112. PMC: 3750293. DOI: 10.1186/1754-6834-6-112. View

10.

Kumar R, Wyman C . Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies. Bioresour Technol. 2009; 100(18):4203-13. DOI: 10.1016/j.biortech.2008.11.057. View

11.

Qing Q, Wyman C . Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover. Biotechnol Biofuels. 2011; 4(1):18. PMC: 3141396. DOI: 10.1186/1754-6834-4-18. View

12.

Jia L, Goncalves G, Takasugi Y, Mori Y, Noda S, Tanaka T . Effect of pretreatment methods on the synergism of cellulase and xylanase during the hydrolysis of bagasse. Bioresour Technol. 2015; 185:158-64. DOI: 10.1016/j.biortech.2015.02.041. View

13.

Li H, Wu H, Xiong L, Chen X, Wang C, Qi G . The hydrolytic efficiency and synergistic action of recombinant xylan-degrading enzymes on xylan isolated from sugarcane bagasse. Carbohydr Polym. 2017; 175:199-206. DOI: 10.1016/j.carbpol.2017.07.075. View

14.

Kim J, Lee Y, Kim T . A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresour Technol. 2015; 199:42-48. DOI: 10.1016/j.biortech.2015.08.085. View

15.

Ding S, Liu Y, Zeng Y, Himmel M, Baker J, Bayer E . How does plant cell wall nanoscale architecture correlate with enzymatic digestibility?. Science. 2012; 338(6110):1055-60. DOI: 10.1126/science.1227491. View

16.

Arantes V, Saddler J . Access to cellulose limits the efficiency of enzymatic hydrolysis: the role of amorphogenesis. Biotechnol Biofuels. 2010; 3:4. PMC: 2844368. DOI: 10.1186/1754-6834-3-4. View

17.

Meng X, Ragauskas A . Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates. Curr Opin Biotechnol. 2014; 27:150-8. DOI: 10.1016/j.copbio.2014.01.014. View

18.

Chen X, Gao Y, Wang L, Chen H, Yan N . Effect of Treatment Methods on Chitin Structure and Its Transformation into Nitrogen-Containing Chemicals. Chempluschem. 2020; 80(10):1565-1572. DOI: 10.1002/cplu.201500326. View

19.

Lou H, Wang M, Lai H, Lin X, Zhou M, Yang D . Reducing non-productive adsorption of cellulase and enhancing enzymatic hydrolysis of lignocelluloses by noncovalent modification of lignin with lignosulfonate. Bioresour Technol. 2013; 146:478-484. DOI: 10.1016/j.biortech.2013.07.115. View

20.

Saha B . Hemicellulose bioconversion. J Ind Microbiol Biotechnol. 2003; 30(5):279-91. DOI: 10.1007/s10295-003-0049-x. View