Coptisine Suppresses Tumor Growth and Progression by Down-regulating MFG-E8 in Colorectal Cancer

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 13

PMID 35548723

Authors

Qianyu Cao

Shengwei Hong

Yuanyuan Li

Heng Chen

Yining Shen

Kang Shao

Mengjie Lu

Hui Dai

Shitang Ma

Guoliang Dai

Affiliations

Soon will be listed here.

Abstract

Treating colorectal cancer (CRC) continues to be a clinical challenge. Coptisine, an alkaloid derived from Franch. shows toxic effects on CRC cells, but its underlying mechanism remains elusive. MFG-E8 is involved in tumor growth and progression. Herein, we evaluated the effects of coptisine on MFG-E8 in CRC, and explored the mechanism. The expression of MFG-E8 in CRC and adjacent normal colon tissue samples from patients was detected. The effects of coptisine on CRC cells HCT116 were evaluated by CCK-8, adhesion and transwell assays. A xenograft tumor model was used to assess the effects of coptisine . The morphology of CRC tissue was observed by HE staining. Cell signaling was tested using western blotting and immunohistochemical assay. The expression of MFG-E8 in human CRC tissue samples significantly increased compared with that of adjacent normal ones. Coptisine significantly reduced the expressions of MFG-E8 in HCT116 cells and tumor-bearing mice. Moreover, coptisine suppressed the growth, adhesion and metastasis of CRC cells. Coptisine also suppressed the expression of MMP-2 and MMP-9 the PI3K/AKT signaling pathway. Furthermore, it inhibited epithelial-mesenchymal transition and . Coptisine inhibited CRC growth and progression by down-regulating MFG-E8, and is a potential candidate for treatment.

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