Effects of Sperm Preparation Techniques on Sperm Survivability and DNA Fragmentation

Overview

Journal J Int Med Res

Publisher Sage Publications

Specialty General Medicine

Date 2022 May 12

PMID 35545843

Authors

Minh Tam Le

Hong Nhan Thi Dang

Trung Van Nguyen

Thai Thanh Thi Nguyen

Quoc Huy Vu Nguyen

Ngoc Thanh Cao

Affiliations

Soon will be listed here.

Abstract

Objective: This study was performed to determine the effect of swim-up (SU) and density gradient centrifugation (DGC) on sperm survival and DNA fragmentation.

Methods: Individual semen samples were analyzed before each was divided into two aliquots (half for SU and half for DGC) for calculation of sperm survival and the DNA fragmentation index (DFI). Sperm DNA fragmentation was determined using the sperm chromatin dispersion test.

Results: The DFI of the 63 semen samples processed using both procedures was lower than that of the fresh semen samples. The DFI was significantly lower for samples processed using the SU than DGC method. In the sperm survival test, the SU technique was associated with increased sperm motility and vitality following preparation. After 24 hours, however, the concentration and percentage of surviving sperm were significantly lower in the SU than DGC group.

Conclusions: Both semen preparation techniques help to minimize sperm DNA fragmentation; however, when the DFI is <30%, the SU technique is more appropriate than DGC. While DGC may be superior for intrauterine insemination, the SU method may be preferable for in vitro fertilization or maturation.

Citing Articles

Sperm Migration and Hyaluronic Acid Binding: Implications for Male Fertility Evaluation.

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Sperm quality metrics were improved by a biomimetic microfluidic selection platform compared to swim-up methods.

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