A Selective and Sensitive Nanosensor for Fluorescent Detection of Specific IgEs to Purified Allergens in Human Serum

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 11

PMID 35542943

Authors

Jianrong Yun

Fang Duan

Liming Liu

Xiulai Chen

Jia Liu

Qiuling Luo

Jing Wu

Affiliations

Soon will be listed here.

Abstract

Food allergies are increasingly recognized as a major healthcare concern. In order to sensitively and specifically detect allergies from blood samples of at-risk allergic patients, an effective magnetic fluorescence sensing platform (EMFP) was constructed. The EMFP incorporated hollow mesoporous silica nanospheres (HMNs) to amplify signal from the target IgE in addition to magnetic nanoparticles (MNPs) to capture and separate the target IgE. The application of EMFP to immunoassays indicated a detection limit of 0.0159 ng mL for low concentration specific immunoglobulin E (sIgE) against purified shellfish (Meta. E.) allergens, which is 15 fold more sensitive than the commercially available Food and Drug Administration-approved analyzers. Notably, EMFP was specific for the targeted sIgE even with interference by other sIgEs. In addition, the detection time is only 75 min, considerably faster than current commercial ELISA kits for IgE assays. Together, these results demonstrated that EMFP has excellent sensitivity and selectivity for the rapid detection of sIgE. The method thus exhibits potential toward the rapid monitoring of sIgE against Meta. E. allergens in clinical application.

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References

Tan Y, Halsey J, Tang T, Wetering S, Taine E, Cleve M . Application of photonic crystal enhanced fluorescence to detection of low serum concentrations of human IgE antibodies specific for a purified cat allergen (Fel D1). Biosens Bioelectron. 2015; 77:194-201. PMC: 4673012. DOI: 10.1016/j.bios.2015.08.071. View

Turkeltaub P, Gergen P . The risk of adverse reactions from percutaneous prick-puncture allergen skin testing, venipuncture, and body measurements: data from the second National Health and Nutrition Examination Survey 1976-80 (NHANES II). J Allergy Clin Immunol. 1989; 84(6 Pt 1):886-90. DOI: 10.1016/0091-6749(89)90384-9. View

Nwaru B, Hickstein L, Panesar S, Muraro A, Werfel T, Cardona V . The epidemiology of food allergy in Europe: a systematic review and meta-analysis. Allergy. 2013; 69(1):62-75. DOI: 10.1111/all.12305. View

Poulsen L, Malling H, Sondergaard I, Weeke B . A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE). J Immunol Methods. 1986; 92(1):131-6. DOI: 10.1016/0022-1759(86)90513-2. View

Du Toit G, Tsakok T, Lack S, Lack G . Prevention of food allergy. J Allergy Clin Immunol. 2016; 137(4):998-1010. DOI: 10.1016/j.jaci.2016.02.005. View

Wang X, Wang P, Fan R, Xu M, Qiang L, Wei L . Solvatochromic and application in dye-sensitized solar cells of sandwich-like Cd(II) complexes: supramolecular architectures based on N(1),N(3)-bis[(6-methoxypyridin-2-yl)methylene]benzene-1,3-diamine. Dalton Trans. 2015; 44(11):5179-90. DOI: 10.1039/c4dt03856h. View

Howanitz P . Errors in laboratory medicine: practical lessons to improve patient safety. Arch Pathol Lab Med. 2005; 129(10):1252-61. DOI: 10.5858/2005-129-1252-EILMPL. View

Sicherer S, Sampson H . Food allergy: Epidemiology, pathogenesis, diagnosis, and treatment. J Allergy Clin Immunol. 2014; 133(2):291-307. DOI: 10.1016/j.jaci.2013.11.020. View

Tsabouri S, Triga M, Makris M, Kalogeromitros D, Church M, Priftis K . Fish and shellfish allergy in children: review of a persistent food allergy. Pediatr Allergy Immunol. 2012; 23(7):608-15. DOI: 10.1111/j.1399-3038.2012.01275.x. View

10.

Sicherer S, Wood R . Allergy testing in childhood: using allergen-specific IgE tests. Pediatrics. 2011; 129(1):193-7. DOI: 10.1542/peds.2011-2382. View

11.

PIPKORN U, Hammarlund A, Enerback L . Prolonged treatment with topical glucocorticoids results in an inhibition of the allergen-induced weal-and-flare response and a reduction in skin mast cell numbers and histamine content. Clin Exp Allergy. 1989; 19(1):19-25. DOI: 10.1111/j.1365-2222.1989.tb02338.x. View

12.

Hamilton R, Williams P . Human IgE antibody serology: a primer for the practicing North American allergist/immunologist. J Allergy Clin Immunol. 2010; 126(1):33-8. DOI: 10.1016/j.jaci.2010.03.014. View

13.

Soler M, Mesa-Antunez P, Estevez M, Ruiz-Sanchez A, Otte M, Sepulveda B . Highly sensitive dendrimer-based nanoplasmonic biosensor for drug allergy diagnosis. Biosens Bioelectron. 2014; 66:115-23. DOI: 10.1016/j.bios.2014.10.081. View

14.

Kowalski M, Ansotegui I, Aberer W, Al-Ahmad M, Akdis M, Ballmer-Weber B . Risk and safety requirements for diagnostic and therapeutic procedures in allergology: World Allergy Organization Statement. World Allergy Organ J. 2016; 9(1):33. PMC: 5062928. DOI: 10.1186/s40413-016-0122-3. View

15.

King E, Vailes L, Tsay A, Satinover S, Chapman M . Simultaneous detection of total and allergen-specific IgE by using purified allergens in a fluorescent multiplex array. J Allergy Clin Immunol. 2007; 120(5):1126-31. DOI: 10.1016/j.jaci.2007.06.043. View

16.

Rona R, Keil T, Summers C, Gislason D, Zuidmeer L, Sodergren E . The prevalence of food allergy: a meta-analysis. J Allergy Clin Immunol. 2007; 120(3):638-46. DOI: 10.1016/j.jaci.2007.05.026. View

17.

Lee K, Blankenship K, McKinney B, Kern G, Buch J, Greenwood J . Proficiency monitoring of monoclonal antibody cocktail-based enzyme-linked immunosorbent assay for detection of allergen-specific immunoglobulin E in dogs. J Vet Diagn Invest. 2015; 27(4):461-9. DOI: 10.1177/1040638715587547. View

18.

Allen K, Koplin J . The epidemiology of IgE-mediated food allergy and anaphylaxis. Immunol Allergy Clin North Am. 2012; 32(1):35-50. DOI: 10.1016/j.iac.2011.11.008. View

19.

Morgan E, Miller Jr W, Wagner B . A comparison of intradermal testing and detection of allergen-specific immunoglobulin E in serum by enzyme-linked immunosorbent assay in horses affected with skin hypersensitivity. Vet Immunol Immunopathol. 2007; 120(3-4):160-7. DOI: 10.1016/j.vetimm.2007.08.007. View

20.

Hu R, Yin Z, Zeng Y, Zhang J, Liu H, Shao Y . A novel biosensor for Escherichia coli O157:H7 based on fluorescein-releasable biolabels. Biosens Bioelectron. 2015; 78:31-36. DOI: 10.1016/j.bios.2015.11.018. View