Thermostable Iron Oxide Nanoparticle Synthesis Within Recombinant Ferritins from the Hyperthermophile CH1

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 11

PMID 35540659

Authors

Jiacheng Yu

Tongwei Zhang

Huangtao Xu

Xiaoli Dong

Yao Cai

Yongxin Pan

Changqian Cao

Affiliations

Soon will be listed here.

Abstract

Thermostable nanoparticles have numerous applications in catalysis and in the oil/gas industry. However, synthesizing these nanoparticles requires expensive polymers. Here, a novel thermostable ferritin named PcFn, originally from the hyperthermophilic archaeon CH1, was overexpressed in , purified and characterized, which could successfully direct the synthesis of thermostable magnetoferritins (M-PcFn) with monodispersed iron oxide nanoparticles in one step. Transmission electron microscopy and magnetic measurements show that the cores of the M-PcFn have an average diameter of 4.7 nm, are well-crystalline and superparamagnetic. Both the PcFn and M-PcFn can resist temperatures up to 110 °C, which is significantly higher than for human H-chain ferritin (HFn) and M-HFn, and comparable to temperatures previously reported for ferritin (PfFn) and M-PfFn. After heating at 110 °C for 30 minutes, PcFn and M-PcFn maintained their secondary structures and PcFn retained 87.4% of its iron uptake activity. This remarkable thermostability of PcFn and M-PcFn suggests potential applications in elevated temperature environments.

Citing Articles

A natural biogenic nanozyme for scavenging superoxide radicals.

Ma L, Zheng J, Zhou N, Zhang R, Fang L, Yang Y Nat Commun. 2024; 15(1):233.

PMID: 38172125 PMC: 10764798. DOI: 10.1038/s41467-023-44463-w.

Magnetite-Based Biosensors and Molecular Logic Gates: From Magnetite Synthesis to Application.

Dudchenko N, Pawar S, Perelshtein I, Fixler D Biosensors (Basel). 2023; 13(3).

PMID: 36979516 PMC: 10046048. DOI: 10.3390/bios13030304.

Hyperthermostable recombinant human heteropolymer ferritin derived from a novel plasmid design.

Srivastava A, Scalcione L, Arosio P, Bou-Abdallah F Protein Sci. 2022; 32(1):e4543.

PMID: 36519270 PMC: 9798250. DOI: 10.1002/pro.4543.

A novel method for visualization of the growth kinetics, structures and behaviours of gas-phase fabricated metallic alloy nanoparticles.

Zhang L, He L, Shi L, Yang Y, Shang G, Hong H RSC Adv. 2022; 10(22):13037-13042.

PMID: 35492094 PMC: 9051413. DOI: 10.1039/d0ra01740j.

Enhanced Magnetic Hyperthermia of Magnetoferritin through Synthesis at Elevated Temperature.

Yu J, Cao C, Fang F, Pan Y Int J Mol Sci. 2022; 23(7).

PMID: 35409372 PMC: 8999155. DOI: 10.3390/ijms23074012.

References

Cai Y, Cao C, He X, Yang C, Tian L, Zhu R . Enhanced magnetic resonance imaging and staining of cancer cells using ferrimagnetic H-ferritin nanoparticles with increasing core size. Int J Nanomedicine. 2015; 10:2619-34. PMC: 4388082. DOI: 10.2147/IJN.S80025. View

Tatur J, Hagen W, Matias P . Crystal structure of the ferritin from the hyperthermophilic archaeal anaerobe Pyrococcus furiosus. J Biol Inorg Chem. 2007; 12(5):615-30. PMC: 1915633. DOI: 10.1007/s00775-007-0212-3. View

Johnson E, Cascio D, Sawaya M, Gingery M, Schroder I . Crystal structures of a tetrahedral open pore ferritin from the hyperthermophilic archaeon Archaeoglobus fulgidus. Structure. 2005; 13(4):637-48. DOI: 10.1016/j.str.2005.01.019. View

Zhang T, Cao C, Tang X, Cai Y, Yang C, Pan Y . Enhanced peroxidase activity and tumour tissue visualization by cobalt-doped magnetoferritin nanoparticles. Nanotechnology. 2016; 28(4):045704. DOI: 10.1088/1361-6528/28/4/045704. View

McDonald I, Thornton J . Satisfying hydrogen bonding potential in proteins. J Mol Biol. 1994; 238(5):777-93. DOI: 10.1006/jmbi.1994.1334. View

Hosein H, Strongin D, Allen M, Douglas T . Iron and cobalt oxide and metallic nanoparticles prepared from ferritin. Langmuir. 2004; 20(23):10283-7. DOI: 10.1021/la0491100. View

Jun X, Lupeng L, Minjuan X, Oger P, Fengping W, Jebbar M . Complete genome sequence of the obligate piezophilic hyperthermophilic archaeon Pyrococcus yayanosii CH1. J Bacteriol. 2011; 193(16):4297-8. PMC: 3147706. DOI: 10.1128/JB.05345-11. View

Fan K, Cao C, Pan Y, Lu D, Yang D, Feng J . Magnetoferritin nanoparticles for targeting and visualizing tumour tissues. Nat Nanotechnol. 2012; 7(7):459-64. DOI: 10.1038/nnano.2012.90. View

Yang R, Chen L, Zhang T, Yang S, Leng X, Zhao G . Self-assembly of ferritin nanocages into linear chains induced by poly(α, L-lysine). Chem Commun (Camb). 2013; 50(4):481-3. DOI: 10.1039/c3cc47847e. View

10.

Lundberg K, Shoemaker D, Adams M, Short J, Sorge J, Mathur E . High-fidelity amplification using a thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene. 1991; 108(1):1-6. DOI: 10.1016/0378-1119(91)90480-y. View

11.

Bagaria H, Yoon K, Neilson B, Cheng V, Lee J, Worthen A . Stabilization of iron oxide nanoparticles in high sodium and calcium brine at high temperatures with adsorbed sulfonated copolymers. Langmuir. 2013; 29(10):3195-206. DOI: 10.1021/la304496a. View

12.

Gromiha M, Oobatake M, Sarai A . Important amino acid properties for enhanced thermostability from mesophilic to thermophilic proteins. Biophys Chem. 1999; 82(1):51-67. DOI: 10.1016/s0301-4622(99)00103-9. View

13.

Argos P, ROSSMAN M, Grau U, Zuber H, FRANK G, Tratschin J . Thermal stability and protein structure. Biochemistry. 1979; 18(25):5698-703. DOI: 10.1021/bi00592a028. View

14.

Fantechi E, Innocenti C, Zanardelli M, Fittipaldi M, Falvo E, Carbo M . A smart platform for hyperthermia application in cancer treatment: cobalt-doped ferrite nanoparticles mineralized in human ferritin cages. ACS Nano. 2014; 8(5):4705-19. DOI: 10.1021/nn500454n. View

15.

Bleicher L, Prates E, Gomes T, Silveira R, Nascimento A, Rojas A . Molecular basis of the thermostability and thermophilicity of laminarinases: X-ray structure of the hyperthermostable laminarinase from Rhodothermus marinus and molecular dynamics simulations. J Phys Chem B. 2011; 115(24):7940-9. DOI: 10.1021/jp200330z. View

16.

Arosio P, Ingrassia R, Cavadini P . Ferritins: a family of molecules for iron storage, antioxidation and more. Biochim Biophys Acta. 2008; 1790(7):589-99. DOI: 10.1016/j.bbagen.2008.09.004. View

17.

Yip K, Stillman T, Britton K, Artymiuk P, Baker P, Sedelnikova S . The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures. Structure. 1995; 3(11):1147-58. DOI: 10.1016/s0969-2126(01)00251-9. View

18.

Vogt G, Woell S, Argos P . Protein thermal stability, hydrogen bonds, and ion pairs. J Mol Biol. 1997; 269(4):631-43. DOI: 10.1006/jmbi.1997.1042. View

19.

Cao C, Wang X, Cai Y, Sun L, Tian L, Wu H . Targeted in vivo imaging of microscopic tumors with ferritin-based nanoprobes across biological barriers. Adv Mater. 2014; 26(16):2566-71. DOI: 10.1002/adma.201304544. View

20.

Gromiha M, Oobatake M, Kono H, Uedaira H, Sarai A . Relationship between amino acid properties and protein stability: buried mutations. J Protein Chem. 1999; 18(5):565-78. DOI: 10.1023/a:1020603401001. View