Selection of Aptamers for AMACR Detection from DNA Libraries with Different Primers

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 11

PMID 35539643

Authors

Deng-Kai Yang

Chia-Fu Chou

Lin-Chi Chen

Affiliations

Soon will be listed here.

Abstract

In this work, we investigated aptamer selection against alpha-methylacyl-CoA racemase (AMACR) with three DNA libraries bearing different primers. Because of increased selection diversity, aptamers with varied structural properties, such as pre-folded, induced-folding, high and low primer-dependent conformations, were discovered. From the selection results, a dimeric aptamer was constructed and capable of detecting over-expression of AMACR in prostate cancer cell lines. In summary, this study demonstrates a library-based approach to obtain aptamers with different binding properties and provides distinct aptamers for flexible design of AMACR detection.

Citing Articles

SELEX: Critical factors and optimization strategies for successful aptamer selection.

Kohlberger M, Gadermaier G Biotechnol Appl Biochem. 2021; 69(5):1771-1792.

PMID: 34427974 PMC: 9788027. DOI: 10.1002/bab.2244.

Inside the Black Box: What Makes SELEX Better?.

Komarova N, Kuznetsov A Molecules. 2019; 24(19).

PMID: 31591283 PMC: 6804172. DOI: 10.3390/molecules24193598.

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