Exploring Selective Autophagy Events in Multiple Biologic Models Using LC3-interacting Regions (LIR)-based Molecular Traps

Overview

Journal Sci Rep

Specialty Science

Date 2022 May 10

PMID 35538106

Authors

Gregoire Quinet

Pierre Genin

Oznur Ozturk

Naima Belgareh-Touze

Lilas Courtot

Renaud Legouis

Robert Weil

Mickael M Cohen

Manuel S Rodriguez

Affiliations

Soon will be listed here.

Abstract

Autophagy is an essential cellular pathway that ensures degradation of a wide range of substrates including damaged organelles or large protein aggregates. Understanding how this proteolytic pathway is regulated would increase our comprehension on its role in cellular physiology and contribute to identify biomarkers or potential drug targets to develop more specific treatments for disease in which autophagy is dysregulated. Here, we report the development of molecular traps based in the tandem disposition of LC3-interacting regions (LIR). The estimated affinity of LC3-traps for distinct recombinant LC3/GABARAP proteins is in the low nanomolar range and allows the capture of these proteins from distinct mammalian cell lines, S. cerevisiae and C. elegans. LC3-traps show preferences for GABARAP/LGG1 or LC3/LGG2 and pull-down substrates targeted to proteaphagy and mitophagy. Therefore, LC3-traps are versatile tools that can be adapted to multiple applications to monitor selective autophagy events in distinct physiologic and pathologic circumstances.

Citing Articles

LIRcentral: a manually curated online database of experimentally validated functional LIR motifs.

Chatzichristofi A, Sagris V, Pallaris A, Eftychiou M, Kalvari I, Price N Autophagy. 2023; 19(12):3189-3200.

PMID: 37530436 PMC: 10621281. DOI: 10.1080/15548627.2023.2235851.

LGG-1/GABARAP lipidation is not required for autophagy and development in .

Leboutet R, Largeau C, Muller L, Prigent M, Quinet G, Rodriguez M Elife. 2023; 12.

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