Sp. Nov., Isolated from Island Soil

A novel Gram-stain-negative, rod-shaped, aerobic and motile bacterium designated strain UL073 was isolated from a forest soil of an island, and subjected to taxonomic characterization. Strain UL073 grew at 10-37 °C (optimum, 30 °C), at pH 5.0-10.0 (optimum, pH 7.0) and in the presence of 0-3 % NaCl (optimum, 0 %), respectively. Strain UL073 showed the highest sequence similarity to PE08 based on 16S rRNA gene analysis with a sequence similarity of 98.08 %, which was well below the suggested cutoff for species distinction. The 16S rRNA gene tree as well as the multilocus sequence analysis and genome-based trees indicated the independent taxonomic position of strain UL073, and the orthologous average nucleotide identity and DNA-DNA hybridization values between strain UL073 and related species were no higher than 84.7 and 28.3% respectively, thus confirming the distinctive taxonomic position of the strain. The chemotaxonomic properties were consistent with those of the genus, as the major fatty acids of the strain were a summed feature consisting of C ω7/C ω6 (31.4 %), another summed feature consisting of C ω7/C ω6 (23.1 %), and C (22.0 %), the major respiratory quinone was ubiquinone 9, and the major polar lipids were phosphatidylethanolamine and diphosphatidylglycerol. The genome size and DNA G+C content of strain UL073 were 4.87 Mbp and 65.9 mol%. On the basis of phenotypic and phylogenetic evidence, strain UL073 should be classified as representing a novel species of , for which the name sp. nov. (type strain=UL073=KCTC 82407=JCM 34511) is proposed.