Cryopreservation Techniques for Ram Sperm

Overview

Journal Vet Med Int

Publisher Wiley

Specialty Veterinary Medicine

Date 2022 May 10

PMID 35535035

Authors

Amit Saha

Mohammad Asaduzzaman

Farida Yeasmin Bari

Affiliations

Soon will be listed here.

Abstract

Germplasm storage and transportation in artificial insemination (AI) and other advanced technologies are facilitated by cryopreservation. In reproduction, the cryopreservation of sperm allows it to be transported across vast distances and used even after the sire's death. However, the technique of cryopreservation might damage sperm and limit their activity. Several cryobiological investigations have reported that the integrity of the sperm membrane is frequently involved in the physical and biological elements that affect sperm survival at low temperatures during the cryopreservation process. However, successful cryopreservation of ram sperm is still a work in progress because a considerable percentage of sperm do not survive the freezing and thawing process. Sperms are destroyed during cryopreservation of semen due to varying concentrations of cryoprotective chemicals and if semen is not cooled at optimal cooling rates. Hence, it is crucial to know the optimum cooling rates with freezing and thawing protocols for maximum recovery of viable and functional sperm cells for a successful cryo-freezing of ram spermatozoa. Therefore, the current study compiled and compared the research on the impact of different cryopreservation procedures, cooling rates, equilibration time, and thawing protocols on post-thaw ram semen quality.

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References

Muino R, Fernandez M, Pena A . Post-thaw survival and longevity of bull spermatozoa frozen with an egg yolk-based or two egg yolk-free extenders after an equilibration period of 18 h. Reprod Domest Anim. 2007; 42(3):305-11. DOI: 10.1111/j.1439-0531.2006.00784.x. View

OConnell M, McClure N, Lewis S . The effects of cryopreservation on sperm morphology, motility and mitochondrial function. Hum Reprod. 2002; 17(3):704-9. DOI: 10.1093/humrep/17.3.704. View

Camara D, Silva S, Almeida F, Nunes J, Guerra M . Effects of antioxidants and duration of pre-freezing equilibration on frozen-thawed ram semen. Theriogenology. 2011; 76(2):342-50. DOI: 10.1016/j.theriogenology.2011.02.013. View

DAlessandro A, Martemucci G . Evaluation of seasonal variations of semen freezability in Leccese ram. Anim Reprod Sci. 2003; 79(1-2):93-102. DOI: 10.1016/s0378-4320(03)00113-1. View

Najafi A, Zhandi M, Towhidi A, Sharafi M, Akbari Sharif A, Motlagh M . Trehalose and glycerol have a dose-dependent synergistic effect on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 2013; 66(3):275-82. DOI: 10.1016/j.cryobiol.2013.03.002. View

Banday M, Lone F, Rasool F, Rashid M, Shikari A . Use of antioxidants reduce lipid peroxidation and improve quality of crossbred ram sperm during its cryopreservation. Cryobiology. 2017; 74:25-30. DOI: 10.1016/j.cryobiol.2016.12.008. View

Ritar A, Ball P, OMay P . Examination of methods for the deep freezing of goat semen. Reprod Fertil Dev. 1990; 2(1):27-34. DOI: 10.1071/rd9900027. View

Songsasen N, Leibo S . Cryopreservation of mouse spermatozoa. II. Relationship between survival after cryopreservation and osmotic tolerance of spermatozoa from three strains of mice. Cryobiology. 1998; 35(3):255-69. DOI: 10.1006/cryo.1997.2047. View

Medeiros C, Forell F, Oliveira A, Rodrigues J . Current status of sperm cryopreservation: why isn't it better?. Theriogenology. 2002; 57(1):327-44. DOI: 10.1016/s0093-691x(01)00674-4. View

10.

Ellis K, Morrison J . Buffers of constant ionic strength for studying pH-dependent processes. Methods Enzymol. 1982; 87:405-26. DOI: 10.1016/s0076-6879(82)87025-0. View

11.

Byrne G, Lonergan P, Wade M, Duffy P, Donovan A, Hanrahan J . Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro. Anim Reprod Sci. 2000; 62(4):265-75. DOI: 10.1016/s0378-4320(00)00121-4. View

12.

Valente S, Pereira R, Baptista M, Marques C, Vasques M, Pereira M . In vitro and in vivo fertility of ram semen cryopreserved in different extenders. Anim Reprod Sci. 2009; 117(1-2):74-7. DOI: 10.1016/j.anireprosci.2009.04.007. View

13.

Paulenz H, Adnoy T, Soderquist L . Comparison of fertility results after vaginal insemination using different thawing procedures and packages for frozen ram semen. Acta Vet Scand. 2007; 49:26. PMC: 2089064. DOI: 10.1186/1751-0147-49-26. View

14.

Mazur P . Cryobiology: the freezing of biological systems. Science. 1970; 168(3934):939-49. DOI: 10.1126/science.168.3934.939. View

15.

Gillan L, Maxwell W, Evans G . Preservation and evaluation of semen for artificial insemination. Reprod Fertil Dev. 2004; 16(4):447-54. DOI: 10.10371/RD04034. View

16.

Giuliano S, Director A, Gambarotta M, Trasorras V, Miragaya M . Collection method, season and individual variation on seminal characteristics in the llama (Lama glama). Anim Reprod Sci. 2007; 104(2-4):359-69. DOI: 10.1016/j.anireprosci.2007.02.016. View

17.

Coyan K, Baspinar N, Bucak M, Akalin P . Effects of cysteine and ergothioneine on post-thawed Merino ram sperm and biochemical parameters. Cryobiology. 2011; 63(1):1-6. DOI: 10.1016/j.cryobiol.2011.04.001. View

18.

Fukui Y, Kohno H, Togari T, Hiwasa M, Okabe K . Fertility after artificial insemination using a soybean-based semen extender in sheep. J Reprod Dev. 2008; 54(4):286-9. DOI: 10.1262/jrd.20004. View

19.

Garcia-Alvarez O, Maroto-Morales A, Ramon M, Del Olmo E, Montoro V, Dominguez-Rebolledo A . Analysis of selected sperm by density gradient centrifugation might aid in the estimation of in vivo fertility of thawed ram spermatozoa. Theriogenology. 2010; 74(6):979-88. DOI: 10.1016/j.theriogenology.2010.04.027. View

20.

Forouzanfar M, Sharafi M, Hosseini S, Ostadhosseini S, Hajian M, Hosseini L . In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 2009; 73(4):480-7. DOI: 10.1016/j.theriogenology.2009.10.005. View