Caβ-subunit Dependence of Forward and Reverse Trafficking of Ca1.2 Calcium Channels

Overview

Journal Mol Brain

Publisher Biomed Central

Specialties Molecular Biology
Neurology

Date 2022 May 9

PMID 35534894

Authors

Laurent Ferron

Sydney D Guderyan

Ethan J Smith

Gerald W Zamponi

Affiliations

Soon will be listed here.

Abstract

Auxiliary Caβ subunits interact with the pore forming Caα subunit to promote the plasma membrane expression of high voltage-activated calcium channels and to modulate the biophysical properties of Ca currents. However, the effect of Caβ subunits on channel trafficking to and from the plasma membrane is still controversial. Here, we have investigated the impact of Caβ1b and Caβ2a subunits on plasma membrane trafficking of Ca1.2 using a live-labeling strategy. We show that the Caβ1b subunit is more potent in increasing Ca1.2 expression at the plasma membrane than the Caβ2a subunit and that this effect is not related to modification of intracellular trafficking of the channel (i.e. neither forward trafficking, nor recycling, nor endocytosis). We conclude that the differential effect of Caβ subunit subtypes on Ca1.2 surface expression is likely due to their differential ability to protect Ca1.2 from degradation.

Citing Articles

Acute phosphatidylinositol 4,5 bisphosphate depletion destabilizes sarcolemmal expression of cardiac L-type Ca channel Ca1.2.

Voelker T, Del Villar S, Westhoff M, Costa A, Coleman A, Hell J Proc Natl Acad Sci U S A. 2023; 120(14):e2221242120.

PMID: 36976770 PMC: 10083594. DOI: 10.1073/pnas.2221242120.

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