Association of Saponin Concentration, Molecular Markers, and Biochemical Factors with Enhancing Resistance to Alfalfa Seedling Damping-off

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2022 May 9

PMID 35531163

Authors

Clara R Azzam

Zeinab M Abd El-Naby

Saieda S Abd El-Rahman

Said A Omar

Esmat F Ali

Ali Majrashi

Mostafa M Rady

Affiliations

Soon will be listed here.

Abstract

Fifteen alfalfa populations were tested for resistance to the seedling damping-off disease sourced by , , and . In a laboratory experiment, saponin treatment significantly diminished the mycelial growth of the causal fungi of alfalfa damping-off disease. Roots of the fifteen alfalfa populations varied in saponin and lignin content. Selection for the considerably resistant plants leads to the best growth performance, desirable yield, and high nutritive values such as crude protein (CP), crude fier (CF), nitrogen free extract (NFE), ash, and ether extract (EE) contents. For the PCR reaction, 10 SSR pairs of the JESPR series primers and the cDNA-SCoT technique with seven primers were used. SSR and SCoT revealed some unique markers that could be linked to resistance to damping-off disease in alfalfa that appeared in the considerably resistant alfalfa population (the promised pop.). SSR and SCoT markers can be an excellent molecular method for judging genetic diversity and germplasm classification in tetraploid alfalfa. We recommend breeding for saponin concentration in the alfalfa plant may affect resistance to some diseases like root rot and damping-off because saponin might improve plant growth, yield, and nutritional values.

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