Untargeted Metabolomics Using Liquid Chromatography Coupled with Mass Spectrometry for Rapid Discovery of Metabolite Biomarkers to Reveal Therapeutic Effects of Seeds Against Osteoporosis

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 9

PMID 35528068

Authors

Fu-Jiang Zhao

Zhao-Bo Zhang

Ning Ma

Xiao Teng

Zhen-Cheng Cai

Ming-Xi Liu

Affiliations

Soon will be listed here.

Abstract

Liquid chromatography coupled with mass spectrometry has been used as metabolomics profiling tool to discover and identify the metabolites in metabolic diseases. Osteoporosis (OP) syndrome is a chronic metabolic disease characterized by bone mass reduction and changes in bone microstructure. Linn. seeds (PCS) have a therapeutic effect on osteoporosis, but their action mechanism and therapeutic target are still unclear. This study aims to explore the metabolic changes of the urine profile in glucocorticoid-induced OP model rats and the therapeutic effect of PCS. High-throughput metabolomics based on the liquid chromatography coupled with mass spectrometry quadrupole time-of-flight mass spectrometry and multivariate data analysis were used to analyze the urine metabolites. The results showed that has an obvious separation between model and control groups. OPLS-DA was used to further analyze and discover substances that contributed to the separation. 42 potential biomarkers and 12 related metabolic pathways were identified in combination with network databases. After the intervention of PCS, 24 biomarkers were significantly regulated, mainly with glycone, serine and threonine metabolism, glutathione metabolism and purine metabolism and other metabolic pathways are related and discovered. This study has proved that PCS has therapeutic effect against OP by regulating that metabolic pathways disturbed in the OP. It provided a basis for the research and future development of new drugs for OP treatment.

Citing Articles

The Gut Microbiome Is Altered in Postmenopausal Women With Osteoporosis and Osteopenia.

Rettedal E, Ilesanmi-Oyelere B, Roy N, Coad J, Kruger M JBMR Plus. 2021; 5(3):e10452.

PMID: 33778322 PMC: 7990138. DOI: 10.1002/jbm4.10452.

References

Sun H, Zhang A, Wang X . Potential role of metabolomic approaches for Chinese medicine syndromes and herbal medicine. Phytother Res. 2012; 26(10):1466-71. DOI: 10.1002/ptr.4613. View

Sun H, Zhang A, Liu S, Qiu S, Li X, Zhang T . Cell metabolomics identify regulatory pathways and targets of magnoline against prostate cancer. J Chromatogr B Analyt Technol Biomed Life Sci. 2018; 1102-1103:143-151. DOI: 10.1016/j.jchromb.2018.10.017. View

Xiong H, Zhang A, Zhao Q, Yan G, Sun H, Wang X . Discovery of quality-marker ingredients of Panax quinquefolius driven by high-throughput chinmedomics approach. Phytomedicine. 2019; 74:152928. DOI: 10.1016/j.phymed.2019.152928. View

Kanis J, Melton 3rd L, Christiansen C, JOHNSTON C, Khaltaev N . The diagnosis of osteoporosis. J Bone Miner Res. 1994; 9(8):1137-41. DOI: 10.1002/jbmr.5650090802. View

Lin H, Zhu H, Tan J, Wang C, Dong Q, Wu F . Comprehensive Investigation on Metabolites of Wild-Simulated American Ginseng Root Based on Ultra-High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry. J Agric Food Chem. 2019; 67(20):5801-5819. DOI: 10.1021/acs.jafc.9b01581. View

Wang M, Chen L, Liu D, Chen H, Tang D, Zhao Y . Metabolomics highlights pharmacological bioactivity and biochemical mechanism of traditional Chinese medicine. Chem Biol Interact. 2017; 273:133-141. DOI: 10.1016/j.cbi.2017.06.011. View

Vidal M, Thibodaux R, Vidal Neira L, Messina O . Osteoporosis: a clinical and pharmacological update. Clin Rheumatol. 2018; 38(2):385-395. DOI: 10.1007/s10067-018-4370-1. View

Zhang A, Sun H, Yan G, Yuan Y, Han Y, Wang X . Metabolomics study of type 2 diabetes using ultra-performance LC-ESI/quadrupole-TOF high-definition MS coupled with pattern recognition methods. J Physiol Biochem. 2013; 70(1):117-28. DOI: 10.1007/s13105-013-0286-z. View

Melton 3rd L, Chrischilles E, Cooper C, Lane A, Riggs B . Perspective. How many women have osteoporosis?. J Bone Miner Res. 1992; 7(9):1005-10. DOI: 10.1002/jbmr.5650070902. View

10.

Wallace B, Saag K, Curtis J, Waljee A . Just the FRAX: Management of Glucocorticoid-Induced Osteoporosis. Gastroenterology. 2018; 154(3):748-750. PMC: 6518402. DOI: 10.1053/j.gastro.2018.01.016. View

11.

Canalis E, Mazziotti G, Giustina A, Bilezikian J . Glucocorticoid-induced osteoporosis: pathophysiology and therapy. Osteoporos Int. 2007; 18(10):1319-28. DOI: 10.1007/s00198-007-0394-0. View

12.

Zhang A, Sun H, Han Y, Yuan Y, Wang P, Song G . Exploratory urinary metabolic biomarkers and pathways using UPLC-Q-TOF-HDMS coupled with pattern recognition approach. Analyst. 2012; 137(18):4200-8. DOI: 10.1039/c2an35780a. View

13.

Zhang H, Zhang A, Zhou X, Sun H, Wang X, Liang L . High-throughput lipidomics reveal mirabilite regulating lipid metabolism as anticancer therapeutics. RSC Adv. 2022; 8(62):35600-35610. PMC: 9087915. DOI: 10.1039/c8ra06190d. View

14.

Chen H, Cao G, Chen D, Wang M, Vaziri N, Zhang Z . Metabolomics insights into activated redox signaling and lipid metabolism dysfunction in chronic kidney disease progression. Redox Biol. 2016; 10:168-178. PMC: 5066525. DOI: 10.1016/j.redox.2016.09.014. View

15.

Fang H, Zhang A, Sun H, Yu J, Wang L, Wang X . High-throughput metabolomics screen coupled with multivariate statistical analysis identifies therapeutic targets in alcoholic liver disease rats using liquid chromatography-mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci. 2019; 1109:112-120. DOI: 10.1016/j.jchromb.2019.01.017. View

16.

Li W, Yan C, Wu Y, Weng Z, Yin F, Yang G . Osteoblasts proliferation and differentiation stimulating activities of the main components of Fructus Psoraleae corylifoliae. Phytomedicine. 2013; 21(4):400-5. DOI: 10.1016/j.phymed.2013.09.015. View

17.

Liu X, Zhang A, Fang H, Li M, Song Q, Su J . Serum metabolomics strategy for understanding the therapeutic effects of Yin-Chen-Hao-Tang against Yanghuang syndrome. RSC Adv. 2022; 8(14):7403-7413. PMC: 9078382. DOI: 10.1039/c7ra11048k. View

18.

Bucay N, Sarosi I, Dunstan C, Morony S, Tarpley J, Capparelli C . osteoprotegerin-deficient mice develop early onset osteoporosis and arterial calcification. Genes Dev. 1998; 12(9):1260-8. PMC: 316769. DOI: 10.1101/gad.12.9.1260. View

19.

Yang B, Zhang A, Sun H, Dong W, Yan G, Li T . Metabolomic study of insomnia and intervention effects of Suanzaoren decoction using ultra-performance liquid-chromatography/electrospray-ionization synapt high-definition mass spectrometry. J Pharm Biomed Anal. 2011; 58:113-24. DOI: 10.1016/j.jpba.2011.09.033. View

20.

Sun H, Zhang A, Song Q, Fang H, Liu X, Su J . Functional metabolomics discover pentose and glucuronate interconversion pathways as promising targets for Yang Huang syndrome treatment with Yinchenhao Tang. RSC Adv. 2022; 8(64):36831-36839. PMC: 9089300. DOI: 10.1039/c8ra06553e. View