Isolation and Characterization of a Clo DF13::Tn901 Plasmid Mutant with Thermosensitive Control of DNA Replication

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Aug 1

PMID 355238

Citations 12

Authors

P M Andreoli

R A Brandsma

E Veltkamp

H J Nijkamp

Affiliations

Soon will be listed here.

Abstract

After nitrosoguanidine mutagenesis, a mutant Escherichia coli strain harboring the Clo DF13::Tn901 plasmid pJN03 was isolated that is thermosensitive (Ts) for growth at 43 degrees C. The mutation responsible for this thermosensitive phenotype resides on the pJN03 plasmid genome. Cells harboring the pJN03 cop-1(Ts) plasmid mutant showed a large increase in plasmid copy number at 43 degrees C accompanied by an increase in the synthesis of plasmid-specified gene products like cloacin DF13 and beta-lactamase. The pJN03 cop-1(Ts) mutant showed uncontrolled plasmid DNA replication at the nonpermissive temperature. Analysis of plasmid deletions showed that the mutation is located in the Clo DF13 map interval from 0 to 12% or 29 to 45%. This implies that native cloacin DF13 and the Clo DF13-specified polypeptides B, C, D, E, and G are not involved in the pleiotropic phenotype of the plasmid mutant pJN03 cop-1(Ts).

Citing Articles

Mutation in the plasmid pUB110 Rep protein affects termination of rolling circle replication.

Bidnenko V, Gruss A, Ehrlich S J Bacteriol. 1993; 175(17):5611-6.

PMID: 8366045 PMC: 206618. DOI: 10.1128/jb.175.17.5611-5616.1993.

Protein H encoded by plasmid Clo DF13 involved in lysis of the bacterial host. II. Functions and regulation of synthesis of the gene H product.

Hakkaart M, Veltkamp E, Nijkamp H Mol Gen Genet. 1981; 183(2):326-32.

PMID: 7035830 DOI: 10.1007/BF00270636.

Mitomycin C-induced synthesis of cloacin DF13 and lethality in cloacinogenic Escherichia coli cells.

Veltkamp E, de Graaf F J Bacteriol. 1981; 146(1):41-8.

PMID: 7012123 PMC: 217049. DOI: 10.1128/jb.146.1.41-48.1981.

Structure and regulation of gene expression of a Clo DF13 plasmid DNA region involved in plasmid segregation and incompatibility.

van den Elzen P, Hakkaart M, van Putten A, Walters H, Veltkamp E, Nijkamp H Nucleic Acids Res. 1983; 11(24):8791-808.

PMID: 6324101 PMC: 326624. DOI: 10.1093/nar/11.24.8791.

Maintenance of the bacteriocinogenic plasmid Clo DF13 in Escherichia coli cells. II. Specific recombination functions involved in plasmid maintenance.

Hakkaart M, Veltkamp E, Nijkamp H Mol Gen Genet. 1982; 188(2):338-44.

PMID: 6296634 DOI: 10.1007/BF00332698.

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