Salmonella Typhimurium Mutants Lacking Protease II

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Aug 1

PMID 355236

Citations 6

Authors

C HEIMAN

C G Miller

Affiliations

Soon will be listed here.

Abstract

Mutants of Salmonella typhimurium lacking protease II, an endoprotease with trypsin-like specificity, have been isolated. These mutants can be identified by using the chromogenic substrate N-methyl-N-p-toluenesulfonyl-L-lysine beta-naphthyl ester to screen colonies growing on agar for the presence of the enzyme. All of the mutations isolated map at locus tlp (typsin-like protease) which is cotransducible (approximately 1%) using phage P1 with tre (trehalose utilization) at approximately 58 min on the Salmonella map. Double mutants lacking both protease I and protease II have been constructed. These strains grew normally. They were able to degrade abnormal proteins and to carry out protein turnover during carbon starvation at the same rate as the wild type.

Citing Articles

Subsite specificity (S3, S2, S1', S2' and S3') of oligopeptidase B from Trypanosoma cruzi and Trypanosoma brucei using fluorescent quenched peptides: comparative study and identification of specific carboxypeptidase activity.

Hemerly J, Oliveira V, Del Nery E, Morty R, Andrews N, Juliano M Biochem J. 2003; 373(Pt 3):933-9.

PMID: 12737623 PMC: 1223545. DOI: 10.1042/BJ20030342.

Substrate recognition properties of oligopeptidase B from Salmonella enterica serovar Typhimurium.

Morty R, Fulop V, Andrews N J Bacteriol. 2002; 184(12):3329-37.

PMID: 12029050 PMC: 135088. DOI: 10.1128/JB.184.12.3329-3337.2002.

Mutations affecting a regulated, membrane-associated esterase in Salmonella typhimurium LT2.

Miller C Mol Gen Genet. 1994; 243(6):674-80.

PMID: 8028584 DOI: 10.1007/BF00279577.

Subcellular distribution of various proteases in Escherichia coli.

Swamy K, GOLDBERG A J Bacteriol. 1982; 149(3):1027-33.

PMID: 7037737 PMC: 216492. DOI: 10.1128/jb.149.3.1027-1033.1982.

Linkage map of Salmonella typhimurium, Edition VI.

Sanderson K, Roth J Microbiol Rev. 1983; 47(3):410-53.

PMID: 6314110 PMC: 281582. DOI: 10.1128/mr.47.3.410-453.1983.

References

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

Davis B . DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964; 121:404-27. DOI: 10.1111/j.1749-6632.1964.tb14213.x. View

Erlanger B, KOKOWSKY N, Cohen W . The preparation and properties of two new chromogenic substrates of trypsin. Arch Biochem Biophys. 1961; 95:271-8. DOI: 10.1016/0003-9861(61)90145-x. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

de Lares L, Ratouchniak J, Casse F . Chromosomal location of gene governing the trehalose utilization in Escherichia coli K12. Mol Gen Genet. 1977; 152(1):105-8. DOI: 10.1007/BF00264946. View

Miller C, Zipser D . Degradation of Escherichia coli beta-galactosidase fragments in protease-deficient mutants of Salmonella typhimurium. J Bacteriol. 1977; 130(1):347-53. PMC: 235212. DOI: 10.1128/jb.130.1.347-353.1977. View

Inouye H, Beckwith J . Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro. Proc Natl Acad Sci U S A. 1977; 74(4):1440-4. PMC: 430790. DOI: 10.1073/pnas.74.4.1440. View

Pacaud M, Sibilli S, Bras G . Protease I from Escherichia coli. Some physicochemical properties and substrate specificity. Eur J Biochem. 1976; 69(1):141-51. DOI: 10.1111/j.1432-1033.1976.tb10867.x. View

Kowit J, Choy W, Champe S, GOLDBERG A . Role and location of "protease I" from Escherichia coli. J Bacteriol. 1976; 128(3):776-84. PMC: 232768. DOI: 10.1128/jb.128.3.776-784.1976. View

10.

Miller C, Mackinnon K . Peptidase mutants of Salmonella typhimurium. J Bacteriol. 1974; 120(1):355-63. PMC: 245770. DOI: 10.1128/jb.120.1.355-363.1974. View

11.

Sanderson K . Linkage map of Salmonella typhimurium, edition IV. Bacteriol Rev. 1972; 36(4):558-86. PMC: 408332. DOI: 10.1128/br.36.4.558-586.1972. View

12.

SCHMIEGER H . Phage P22-mutants with increased or decreased transduction abilities. Mol Gen Genet. 1972; 119(1):75-88. DOI: 10.1007/BF00270447. View

13.

Sanderson K, Ross H, Ziegler L, Makela P . F + , Hfr, and F' strains of Salmonella typhimurium and Salmonella abony. Bacteriol Rev. 1972; 36(4):608-37. PMC: 408334. DOI: 10.1128/br.36.4.608-637.1972. View

14.

Regnier P, THANG M . Subcellular distribution and characterization of endo and exo-cellular proteases in E. coli. Biochimie. 1972; 54(10):1227-36. DOI: 10.1016/s0300-9084(72)80063-4. View

15.

Enomoto M, Stocker B . Transduction by phage P1kc in Salmonella typhimurium. Virology. 1974; 60(2):503-14. DOI: 10.1016/0042-6822(74)90344-4. View

16.

GOLDBERG A . Degradation of abnormal proteins in Escherichia coli (protein breakdown-protein structure-mistranslation-amino acid analogs-puromycin). Proc Natl Acad Sci U S A. 1972; 69(2):422-6. PMC: 426471. DOI: 10.1073/pnas.69.2.422. View

17.

Pacaud M, Richaud C . Protease II from Escherichia coli. Purification and characterization. J Biol Chem. 1975; 250(19):7771-9. View

18.

Pine M . Turnover of intracellular proteins. Annu Rev Microbiol. 1972; 26:103-26. DOI: 10.1146/annurev.mi.26.100172.000535. View

19.

Pacaud M, Uriel J . Isolation and some propeties of a proteolytic enzyme from Escherichia coli (protease I). Eur J Biochem. 1971; 23(3):435-42. DOI: 10.1111/j.1432-1033.1971.tb01638.x. View

20.

Meiss H, Brill W, MAGASANIK B . Genetic control of histidine degradation in Salmonella typhimurium, strain LT-2. J Biol Chem. 1969; 244(19):5382-91. View