Regulation of the Synthesis of Ribonucleoside Diphosphate Reductase in Escherichia Coli: Specific Activity of the Enzyme in Relationship to Perturbations of DNA Replication

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1978 Aug 1

PMID 355225

Citations 18

Authors

D Filpula

J A Fuchs

Affiliations

Soon will be listed here.

Abstract

Ribonucleoside diphosphate reductase (RDP reductase) activity was found to greatly increase after a shift to the nonpermissive temperature in Escherichia coli mutants temperature sensitive for DNA elongation (dnaE dnaG dnaZ lig) or DNA initiation (dnaA dnaC dnaI). However, the kinetics of increase in RDP reductase after a shift to nonpermissive conditions were significantly different in initiation-defective mutants compared with elongation-defective mutants. In strains without defects in DNA metabolism, the specific activity of RDP reductase was found to increase with increasing growth rate. Nutritional shifts to faster growth conditions caused cells to transiently overproduce RDP reductase before adjusting to the new steady-state conditions.

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References

Davis B, MINGIOLI E . Mutants of Escherichia coli requiring methionine or vitamin B12. J Bacteriol. 1950; 60(1):17-28. PMC: 385836. DOI: 10.1128/jb.60.1.17-28.1950. View

Goss W, Deitz W, Cook T . MECHANISM OF ACTION OF NALIDIXIC ACID ON ESCHERICHIA COLI.II. INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS. J Bacteriol. 1965; 89:1068-74. PMC: 277597. DOI: 10.1128/jb.89.4.1068-1074.1965. View

Pritchard R, Lark K . INDUCTION OF REPLICATION BY THYMINE STARVATION AT THE CHROMOSOME ORIGIN IN ESCHERICHIA COLI. J Mol Biol. 1964; 9:288-307. DOI: 10.1016/s0022-2836(64)80208-4. View

Lark K, Repko T, Hoffman E . THE EFFECT OF AMINO ACID DEPRIVATION ON SUBSEQUENT DEOXYRIBONUCLEIC ACID REPLICATION. Biochim Biophys Acta. 1963; 76:9-24. View

MAALOE O, HANAWALT P . Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961; 3:144-55. DOI: 10.1016/s0022-2836(61)80041-7. View

Luria S, BURROUS J . Hybridization between Escherichia coli and Shigella. J Bacteriol. 1957; 74(4):461-76. PMC: 289941. DOI: 10.1128/jb.74.4.461-476.1957. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Fuchs J . Coordinate control of the synthesis of ribonucleoside diphosphate reductase components in Escherichia coli. J Bacteriol. 1977; 130(2):957-9. PMC: 235305. DOI: 10.1128/jb.130.2.957-959.1977. View

Filpula D, Fuchs J . Regulation of ribonucleoside diphosphate reductase synthesis in Escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis. J Bacteriol. 1977; 130(1):107-13. PMC: 235179. DOI: 10.1128/jb.130.1.107-113.1977. View

10.

Eriksson S, SJOBERG B, Hahne S . Ribonucleoside diphosphate reductase from Escherichia coli. An immunological assay and a novel purification from an overproducing strain lysogenic for phage lambdadnrd. J Biol Chem. 1977; 252(17):6132-8. View

11.

Chu H, Malone M, Haldenwang W, Walker J . Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures. J Bacteriol. 1977; 132(1):151-8. PMC: 221839. DOI: 10.1128/jb.132.1.151-158.1977. View

12.

BRUNSCHEDE H, Dove T, Bremer H . Establishment of exponential growth after a nutritional shift-up in Escherichia coli B/r: accumulation of deoxyribonucleic acid, ribonucleic acid, and protein. J Bacteriol. 1977; 129(2):1020-33. PMC: 235042. DOI: 10.1128/jb.129.2.1020-1033.1977. View

13.

CORY J, Mansell M, Whitford Jr T . Control of ribonucleotide reductase in mammalian cells. Adv Enzyme Regul. 1976; 14:45-62. DOI: 10.1016/0065-2571(76)90007-8. View

14.

Biswas C, Hardy J, Beck W . Release of repressor control of ribonucleotide reductase by thymine starvation. J Biol Chem. 1965; 240(9):3631-40. View

15.

Vosberg H, HOFFMANN-BERLING H . DNA synthesis in nucleotide-permeable Escherichia coli cells. I. Preparation and properties of ether-treated cells. J Mol Biol. 1971; 58(3):739-53. DOI: 10.1016/0022-2836(71)90037-4. View

16.

Gottesman M, Hicks M, Gellert M . Genetics and function of DNA ligase in Escherichia coli. J Mol Biol. 1973; 77(4):531-47. DOI: 10.1016/0022-2836(73)90221-0. View

17.

Ricard M, Hirota Y . [Effects of salts on the process of cell division in E. coli]. C R Acad Hebd Seances Acad Sci D. 1969; 268(9):1335-8. View

18.

Hartree E . Determination of protein: a modification of the Lowry method that gives a linear photometric response. Anal Biochem. 1972; 48(2):422-7. DOI: 10.1016/0003-2697(72)90094-2. View

19.

Lane H, Denhardt D . The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome. J Bacteriol. 1974; 120(2):805-14. PMC: 245842. DOI: 10.1128/jb.120.2.805-814.1974. View

20.

Filip C, Allen J, Gustafson R, Allen R, Walker J . Bacterial cell division regulation: characterization of the dnaH locus of Escherichia coli. J Bacteriol. 1974; 119(2):443-9. PMC: 245626. DOI: 10.1128/jb.119.2.443-449.1974. View