Improvement of High-glucose and Insulin Resistance of Chromium Malate in 3T3-L1 Adipocytes by Glucose Uptake and Insulin Sensitivity Signaling Pathways and Its Mechanism

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35521592

Authors

Weiwei Feng

Yongchao Liu

Fan Fei

Yao Chen

Yangyang Ding

Mengjiao Yan

Yun Feng

Ting Zhao

Guanghua Mao

Liuqing Yang

Xiangyang Wu

Affiliations

Soon will be listed here.

Abstract

Previous study has revealed that chromium malate could improve insulin resistance and the regulation of fasting blood glucose in type 2 diabetic rats. This study was designed to investigate the effect of chromium malate on hypoglycemic and improve insulin resistance activities in 3T3-L1 adipocytes with insulin resistance and investigate the acting mechanism. The result indicated that chromium malate exhibited direct hypoglycemic activity . Compared with the model group, chromium malate could significantly promote the expression levels of GLUT-4, Akt, Irs-1, PPARγ, PI3K and p38-MAPK and their mRNA, increase -AKT/AKT level, AKT and AMPKβ1 phosphorylation and reduce Irs-1 phosphorylation and -Irs-1/Irs-1 level in 3T3-L1 adipocytes ( < 0.05). Chromium malate is more effective in regulating the proteins and mRNA expressions than those of chromium trichloride and chromium picolinate. Compared to the model group, pretreatment with the specific p38-MAPK inhibitor completely inhibited the GLUT-4 and Irs-1 proteins and mRNA expressions induced by the chromium malate. In conclusion, chromium malate had a beneficial influence on improvement of controlling glucose levels and insulin resistance in 3T3-L1 adipocytes with insulin resistance by regulating proteins productions and genes expressions in glucose uptake and insulin sensitivity signaling pathways.

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References

Stattermayer A, Traussnigg S, Aigner E, Kienbacher C, Huber-Schonauer U, Steindl-Munda P . Low hepatic copper content and PNPLA3 polymorphism in non-alcoholic fatty liver disease in patients without metabolic syndrome. J Trace Elem Med Biol. 2016; 39:100-107. DOI: 10.1016/j.jtemb.2016.08.006. View

Akhlaghi F, Matson K, Mohammadpour A, Kelly M, Karimani A . Clinical Pharmacokinetics and Pharmacodynamics of Antihyperglycemic Medications in Children and Adolescents with Type 2 Diabetes Mellitus. Clin Pharmacokinet. 2016; 56(6):561-571. PMC: 5425330. DOI: 10.1007/s40262-016-0472-6. View

Guariguata L, Whiting D, Hambleton I, Beagley J, Linnenkamp U, Shaw J . Global estimates of diabetes prevalence for 2013 and projections for 2035. Diabetes Res Clin Pract. 2014; 103(2):137-49. DOI: 10.1016/j.diabres.2013.11.002. View

Feng W, Zhao T, Mao G, Wang W, Feng Y, Li F . Type 2 diabetic rats on diet supplemented with chromium malate show improved glycometabolism, glycometabolism-related enzyme levels and lipid metabolism. PLoS One. 2015; 10(5):e0125952. PMC: 4420285. DOI: 10.1371/journal.pone.0125952. View

Park P, Cho J, Cho E . Specific visible radiation facilitates lipolysis in mature 3T3-L1 adipocytes via rhodopsin-dependent β3-adrenergic signaling. Eur J Cell Biol. 2017; 96(4):301-311. DOI: 10.1016/j.ejcb.2017.03.015. View

Yang X, Palanichamy K, Ontko A, Rao M, Fang C, Ren J . A newly synthetic chromium complex--chromium(phenylalanine)3 improves insulin responsiveness and reduces whole body glucose tolerance. FEBS Lett. 2005; 579(6):1458-64. DOI: 10.1016/j.febslet.2005.01.049. View

Wang Y, Yao M . Effects of chromium picolinate on glucose uptake in insulin-resistant 3T3-L1 adipocytes involve activation of p38 MAPK. J Nutr Biochem. 2009; 20(12):982-91. DOI: 10.1016/j.jnutbio.2008.09.002. View

Dhanya R, Arya A, Nisha P, Jayamurthy P . Quercetin, a Lead Compound against Type 2 Diabetes Ameliorates Glucose Uptake via AMPK Pathway in Skeletal Muscle Cell Line. Front Pharmacol. 2017; 8:336. PMC: 5462925. DOI: 10.3389/fphar.2017.00336. View

Wu X, Li F, Xu W, Zhao J, Zhao T, Liang L . Anti-hyperglycemic activity of chromium(III) malate complex in alloxan-induced diabetic rats. Biol Trace Elem Res. 2010; 143(2):1031-43. DOI: 10.1007/s12011-010-8916-6. View

10.

Ortega A, Berna G, Rojas A, Martin F, Soria B . Gene-Diet Interactions in Type 2 Diabetes: The Chicken and Egg Debate. Int J Mol Sci. 2017; 18(6). PMC: 5486011. DOI: 10.3390/ijms18061188. View

11.

Lee S, Lai F, Shi L, Chou Y, Yen I, Chang T . Rhodiola crenulata extract suppresses hepatic gluconeogenesis via activation of the AMPK pathway. Phytomedicine. 2015; 22(4):477-86. DOI: 10.1016/j.phymed.2015.01.016. View

12.

Liu Y, Song A, Zang S, Wang C, Song G, Li X . Jinlida reduces insulin resistance and ameliorates liver oxidative stress in high-fat fed rats. J Ethnopharmacol. 2015; 162:244-52. DOI: 10.1016/j.jep.2014.12.040. View

13.

Song E, Lee Y, Kim Y, Yeom J, Yoo C, Kim H . NAADP mediates insulin-stimulated glucose uptake and insulin sensitization by PPARγ in adipocytes. Cell Rep. 2012; 2(6):1607-19. DOI: 10.1016/j.celrep.2012.10.018. View

14.

Xia X, Xiang X, Huang F, Zheng M, Cong R, Han L . Dietary polyphenol canolol from rapeseed oil attenuates oxidative stress-induced cell damage through the modulation of the p38 signaling pathway. RSC Adv. 2022; 8(43):24338-24345. PMC: 9082107. DOI: 10.1039/c8ra04130j. View

15.

Tsave O, Yavropoulou M, Kafantari M, Gabriel C, Yovos J, Salifoglou A . The adipogenic potential of Cr(III). A molecular approach exemplifying metal-induced enhancement of insulin mimesis in diabetes mellitus II. J Inorg Biochem. 2016; 163:323-331. DOI: 10.1016/j.jinorgbio.2016.07.015. View

16.

Fang Z, Zhao M, Zhen H, Chen L, Shi P, Huang Z . Genotoxicity of tri- and hexavalent chromium compounds in vivo and their modes of action on DNA damage in vitro. PLoS One. 2014; 9(8):e103194. PMC: 4128586. DOI: 10.1371/journal.pone.0103194. View

17.

Mohd Fauzi F, John C, Karunanidhi A, Mussa H, Ramasamy R, Adam A . Understanding the mode-of-action of Cassia auriculata via in silico and in vivo studies towards validating it as a long term therapy for type II diabetes. J Ethnopharmacol. 2016; 197:61-72. DOI: 10.1016/j.jep.2016.07.058. View

18.

Manygoats K, Yazzie M, Stearns D . Ultrastructural damage in chromium picolinate-treated cells: a TEM study. Transmission electron microscopy. J Biol Inorg Chem. 2002; 7(7-8):791-8. DOI: 10.1007/s00775-002-0357-z. View

19.

Brooks M, Grimes J, Lloyd K, Krafka K, Lamptey A, Spears J . Chromium propionate in broilers: effect on insulin sensitivity. Poult Sci. 2016; 95(5):1096-104. DOI: 10.3382/ps/pew018. View

20.

Mao G, Ren Y, Feng W, Li Q, Wu H, Jin D . Antitumor and immunomodulatory activity of a water-soluble polysaccharide from Grifola frondosa. Carbohydr Polym. 2015; 134:406-12. DOI: 10.1016/j.carbpol.2015.08.020. View