Development of a Flexible, Sweat-based Neuropeptide Y Detection Platform

Overview

Journal RSC Adv

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 May 6

PMID 35520310

Authors

Nathan Kodjo Mintah Churcher

Sayali Upasham

Paul Rice

Serena Bhadsavle

Shalini Prasad

Affiliations

Soon will be listed here.

Abstract

Neuropeptide Y (NPY) biomarker levels have a close association with the diagnosis of Major Depression Disorder (MDD) and anxiety disorders. Quantifying NPY in correlation to self-reported symptoms will be an important measure to ensure a relatively uniform diagnosis and help with disease prognosis of these disorders. The work presented is a novel, passive eccrine sweat based, electrochemical detection platform for quantification of NPY biomarker levels. The paper offers a comparison between non-porous and porous sensor platforms using various electrochemical detection techniques. This work uses a novel strategy towards designing an optimal nanobioelectronic interface to measure NPY. The portability aspect of this detection platform is discussed by the demonstration a novel, portable EmStat Pico based electronic platform. The detection limit of the sensor is 10 pg mL and its range is 20-500 pg mL. The NPY detection platform is envisioned to be a wearable point-of need monitoring system for management of chronic anxiety disorders and MDD.

Citing Articles

Wearable Electrochemical Biosensors for Advanced Healthcare Monitoring.

Duan H, Peng S, He S, Tang S, Goda K, Wang C Adv Sci (Weinh). 2024; 12(2):e2411433.

PMID: 39588557 PMC: 11727287. DOI: 10.1002/advs.202411433.

Development of a Neuropeptide Y-Sensitive Implantable Microelectrode for Continuous Measurements.

Fernandez-Vega L, Melendez-Rodriguez D, Ospina-Alejandro M, Casanova K, Vazquez Y, Cunci L ACS Sens. 2024; 9(5):2645-2652.

PMID: 38709872 PMC: 11127761. DOI: 10.1021/acssensors.4c00449.

Measurement of Neuropeptide Y in Aptamer-Modified Planar Electrodes.

Lopez L, Martinez L, Caicedo J, Fernandez-Vega L, Cunci L Electrochim Acta. 2024; 488.

PMID: 38654828 PMC: 11034791. DOI: 10.1016/j.electacta.2024.144243.

Measurement of neuropeptide Y with molecularly imprinted polypyrrole on carbon fiber microelectrodes.

Lopez L, Lozano K, Cruz J, Flores K, Fernandez-Vega L, Cunci L Neuropeptides. 2024; 104:102413.

PMID: 38335798 PMC: 10940184. DOI: 10.1016/j.npep.2024.102413.

Development of an Electrochemical, Aptamer-Based Sensor for Dynamic Detection of Neuropeptide Y.

Seibold J, Abeykoon S, Ross A, White R ACS Sens. 2023; 8(12):4504-4511.

PMID: 38033269 PMC: 11214579. DOI: 10.1021/acssensors.3c00855.

References

Thomas L, Scheidt H, Bettio A, Beck-Sickinger A, Huster D, Zschornig O . The interaction of neuropeptide Y with negatively charged and zwitterionic phospholipid membranes. Eur Biophys J. 2009; 38(5):663-77. DOI: 10.1007/s00249-009-0423-3. View

Xiao X, Kuang Z, Burke B, Chushak Y, Farmer B, Mirau P . In Silico Discovery and Validation of Neuropeptide-Y-Binding Peptides for Sensors. J Phys Chem B. 2019; 124(1):61-68. DOI: 10.1021/acs.jpcb.9b09439. View

Ohhashi T, Sakaguchi M, Tsuda T . Human perspiration measurement. Physiol Meas. 1998; 19(4):449-61. DOI: 10.1088/0967-3334/19/4/001. View

Sanghavi B, Varhue W, Chavez J, Chou C, Swami N . Electrokinetic preconcentration and detection of neuropeptides at patterned graphene-modified electrodes in a nanochannel. Anal Chem. 2014; 86(9):4120-5. DOI: 10.1021/ac500155g. View

Bhide A, Muthukumar S, Saini A, Prasad S . Simultaneous lancet-free monitoring of alcohol and glucose from low-volumes of perspired human sweat. Sci Rep. 2018; 8(1):6507. PMC: 5916883. DOI: 10.1038/s41598-018-24543-4. View

Patterson M, Galloway S, Nimmo M . Variations in regional sweat composition in normal human males. Exp Physiol. 2001; 85(6):869-75. DOI: 10.1111/j.1469-445x.2000.02058.x. View

Ronkainen N, Halsall H, Heineman W . Electrochemical biosensors. Chem Soc Rev. 2010; 39(5):1747-63. DOI: 10.1039/b714449k. View

Munje R, Muthukumar S, Jagannath B, Prasad S . A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs). Sci Rep. 2017; 7(1):1950. PMC: 5434046. DOI: 10.1038/s41598-017-02133-0. View

Krstenansky J, Buck S . The synthesis, physical characterization and receptor binding affinity of neuropeptide Y (NPY). Neuropeptides. 1987; 10(1):77-85. DOI: 10.1016/0143-4179(87)90091-6. View

10.

Cizza G, Marques A, Eskandari F, Christie I, Torvik S, Silverman M . Elevated neuroimmune biomarkers in sweat patches and plasma of premenopausal women with major depressive disorder in remission: the POWER study. Biol Psychiatry. 2008; 64(10):907-11. PMC: 2610843. DOI: 10.1016/j.biopsych.2008.05.035. View

11.

Rose D, Ratterman M, Griffin D, Hou L, Kelley-Loughnane N, Naik R . Adhesive RFID Sensor Patch for Monitoring of Sweat Electrolytes. IEEE Trans Biomed Eng. 2014; 62(6):1457-65. DOI: 10.1109/TBME.2014.2369991. View

12.

Upasham S, Tanak A, Jagannath B, Prasad S . Development of ultra-low volume, multi-bio fluid, cortisol sensing platform. Sci Rep. 2018; 8(1):16745. PMC: 6233171. DOI: 10.1038/s41598-018-35199-5. View

13.

Hart J, Pemberton R, Luxton R, Wedge R . Studies towards a disposable screen-printed amperometric biosensor for progesterone. Biosens Bioelectron. 1997; 12(11):1113-21. DOI: 10.1016/s0956-5663(97)00033-x. View

14.

Radha Shanmugam N, Panneer Selvam A, Barrett T, Kazmierczak S, Rana M, Prasad S . Portable nanoporous electrical biosensor for ultrasensitive detection of Troponin-T. Future Sci OA. 2016; 1(3):FSO24. PMC: 5137999. DOI: 10.4155/fso.15.22. View

15.

Munje R, Muthukumar S, Panneer Selvam A, Prasad S . Flexible nanoporous tunable electrical double layer biosensors for sweat diagnostics. Sci Rep. 2015; 5:14586. PMC: 4588585. DOI: 10.1038/srep14586. View

16.

Dyck M, Losche M . Interaction of the neurotransmitter, neuropeptide Y, with phospholipid membranes: film balance and fluorescence microscopy studies. J Phys Chem B. 2006; 110(44):22143-51. PMC: 2553689. DOI: 10.1021/jp056697y. View

17.

Abdallah M, Buchanan-Vega J, Lee K, Wenner B, Allen J, Allen M . Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors. Sensors (Basel). 2019; 20(1). PMC: 6982811. DOI: 10.3390/s20010126. View

18.

Upasham S, Thai K, Muthyala R, Prasad S . Flexible, low volume detection of chronobiology biomarkers from human sweat. Analyst. 2019; 145(3):784-796. DOI: 10.1039/c9an01968e. View

19.

Bae J, Han J, Chung T . Electrochemistry at nanoporous interfaces: new opportunity for electrocatalysis. Phys Chem Chem Phys. 2011; 14(2):448-63. DOI: 10.1039/c1cp22927c. View

20.

Crespi F, Dalessandro D, Annovazzi-Lodi V, Heidbreder C, Norgia M . In vivo voltammetry: from wire to wireless measurements. J Neurosci Methods. 2004; 140(1-2):153-61. DOI: 10.1016/j.jneumeth.2004.06.018. View